Renal Na-K-Cl cotransporter NKCC2 in Dahl salt-sensitive rats

Journal of Hypertension
Miriam Alvarez-Guerra, R P Garay

Abstract

Dahl salt-sensitive (DS) rats are characterized by enhanced NaCl reabsorption in the loop of Henle, but the responsible ion transport protein is unknown. To investigate renal Na-K-Cl cotransporter NKCC2 function and expression in DS rats under a low-salt diet. NKCC2 functioning was assessed in vitro by measuring bumetanide-sensitive rubidium uptake and cytosolic chloride concentration in isolated medullary thick ascending limb (mTAL) tubules, and in vivo by measuring the salidiuretic action of orally given bumetanide. NKCC2 expression was assessed by Western blot analysis of outer medullary proteins using T4 monoclonal antibody. mTAL tubules from DS rats exhibited significantly higher bumetanide-sensitive rubidium uptake (85.1 +/- 4.8 versus 66.2 +/- 4.4 nmol/min per mg protein in DS and DR, (Dahl salt-resistant) rats, respectively; P = 0.011) and significantly higher cytosolic chloride (32.8 +/- 1.7 versus 25.0 +/- 1.5 mmol/l in DS and DR rats, respectively). Moreover, DS rats showed a significantly higher (P < 0.001) natriuretic response to bumetanide (1.13 +/- 0.05 versus 0.64 +/- 0.09 mmole/3 h in DS and DR rats, respectively). Finally, Western blot analysis revealed less NKCC2 expression in DS rats. We conclude that DS rat...Continue Reading

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Citations

May 10, 2011·Pflügers Archiv : European journal of physiology·Monica CarmosinoGiovanna Valenti
Sep 24, 2010·Molecular Biology of the Cell·Monica CarmosinoMaria Svelto
Mar 23, 2002·Journal of Hypertension·Théophile Godfraind
Nov 4, 2006·Journal of Hypertension·Rosemary WangensteenMiriam Alvarez-Guerra
Nov 4, 2006·Journal of Hypertension·Nick Ashton
Jan 12, 2010·Current Opinion in Nephrology and Hypertension·Sergei N OrlovPavel Hamet
Jun 21, 2008·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·S Randall ThomasRonald J White
Sep 9, 2011·American Journal of Physiology. Renal Physiology·Gustavo R AresPablo A Ortiz
Feb 11, 2011·American Journal of Physiology. Renal Physiology·Mohammed Ziaul HaquePablo Alfredo Ortiz
Jan 14, 2010·Nephron. Physiology·Cristina Esteva-FontPatricia Fernández-Llama
Jan 4, 2012·Biology of the Cell·Monica CarmosinoMaria Svelto
Jan 24, 2006·Biochemical and Biophysical Research Communications·Wataru AoiYoshinori Marunaka
Feb 27, 2004·Biochemical and Biophysical Research Communications·Wataru AoiYoshinori Marunaka
Feb 18, 2004·Hypertension·Kimberly M HoaglandRichard J Roman
Feb 18, 2004·Hypertension·Abraham AvivAlan Weder
Aug 19, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Sergei N Orlov, Alexander A Mongin
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