PMID: 12763768May 24, 2003Paper

Renal Na,K-ATPase structure from cryo-electron microscopy of two-dimensional crystals

Annals of the New York Academy of Sciences
H HebertA B Maunsbach

Abstract

The molecular structure of Na,K-ATPase was determined by electron crystallography from two-dimensional crystals induced in purified membranes isolated from the outer medulla of pig kidney. The P2 type unit cell contains two protomers in the E(2) conformation, each of them with a size of 65 x 75 x 150 A(3). The alpha, beta, and gamma subunits in the membrane crystals were demonstrated in the crystals with Western blotting and related to distinct domains in the density map. The alpha subunit corresponds to most of the density in the transmembrane region as well as to the large hydrophilic headpiece on the cytoplasmic side of the membrane. The headpiece is divided into three separated domains. One of these gives rise to an elongated projection onto the membrane plane, while the putative nucleotide binding and phosphorylation domains form compact densities in the rest of the cytoplasmic part of the structure. Density on the extracellular face corresponds to the protein part of the beta subunit. Ten helices from the catalytic a subunit correspond to two groups of distinct densities in the transmembrane region. The structure of the lipid bilayer spanning part also suggests positions for the transmembrane helices from the beta and gam...Continue Reading

References

Jan 1, 1990·Progress in Nucleic Acid Research and Molecular Biology·J B LingrelE M Price
Jun 1, 1988·Journal of Ultrastructure and Molecular Structure Research·M SöderholmA B Maunsbach
Jul 1, 1988·Journal of Ultrastructure and Molecular Structure Research·H HebertA B Maunsbach
Aug 31, 1981·FEBS Letters·E SkriverP L Jørgensen
Aug 12, 1982·Biochimica Et Biophysica Acta·H HebertA B Maunsbach
May 6, 1996·Biochimica Et Biophysica Acta·J V MøllerM le Maire
Jan 1, 1996·Journal of Structural Biology·R A CrowtherJ M Smith
Jan 24, 1998·The Journal of Biological Chemistry·A G TherienR Blostein
Sep 8, 1998·Current Opinion in Structural Biology·W KühlbrandtG A Scarborough
May 1, 1999·Journal of Structural Biology·W WriggersJ A McCammon
Nov 24, 1999·The Journal of Biological Chemistry·E ArystarkhovaK J Sweadner
Feb 7, 2002·Journal of Molecular Biology·Chen XuDavid L Stokes
Aug 9, 2002·Nature·Chikashi Toyoshima, Hiromi Nomura

❮ Previous
Next ❯

Citations

Jan 31, 2012·Theriogenology·Katie D Hickey, Mary M Buhr

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.