Renal nitric oxide production in rat pregnancy: role of constitutive nitric oxide synthases.

American Journal of Physiology. Renal Physiology
Cheryl A SmithC Baylis

Abstract

Functional studies show that increased renal nitric oxide (NO) mediates the renal vasodilation and increased glomerular filtration rate that occur during normal pregnancy. We investigated whether changes in the constitutive NO synthases (NOS), endothelial (eNOS) and neuronal (nNOS), were associated with the increased renal NO production in normal midterm pregnancy in the rat. In kidneys from midterm pregnant (MP: 11-13 days gestation), late-term pregnant (LP: 18-20 days gestation), and similarly aged virgin (V) rats, transcript and protein abundance for eNOS and the nNOSα and nNOSβ splice variants, as well as the rate of L-arginine-to-L-citrulline conversion, were determined as a measure of NOS activity. At MP, renal cortical abundance of the total eNOS protein and phosphorylated (Ser(1177)) eNOS was reduced, and L-arginine-to-L-citrulline conversion in the cortical membrane fraction was decreased; these declines were also seen in LP. There were no changes in the eNOS transcript. In contrast, L-arginine-to-L-citrulline conversion in the soluble fraction of renal cortex increased at MP and then declined at LP. This MP increase was ablated by S-methylthiocitrulline, a nNOS inhibitor. Using Western blotting, we did not detect a ch...Continue Reading

References

Aug 1, 1980·Kidney International·J M Davison, W Dunlop
Jun 1, 1994·Baillière's Clinical Obstetrics and Gynaecology·C Baylis
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·M J EliassonS H Snyder
Mar 27, 1997·Biochemical and Biophysical Research Communications·R SinghG Chaudhuri
Nov 14, 1998·The American Journal of Physiology·A HuberH D Allescher
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y WangP A Marsden
Mar 16, 2001·American Journal of Physiology. Renal Physiology·M A CadnapaphornchaiR W Schrier
May 16, 2001·American Journal of Physiology. Renal Physiology·S XiaoC Baylis
Jul 21, 2001·The Biochemical Journal·W K AldertonR G Knowles
Oct 20, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·S R AbramJ P Granger
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 8, 2002·Hypertension·Barbara T AlexanderJoey P Granger
Mar 25, 2004·American Journal of Physiology. Renal Physiology·Aaron ErdelyChris Baylis
Jun 29, 2004·Journal of the Society for Gynecologic Investigation·Jacqueline NovakKirk P Conrad
Feb 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·K Joseph HurtArthur L Burnett
Dec 17, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Cheryl SmithChris Baylis

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Citations

Oct 6, 2011·Current Hypertension Reports·Kirk P Conrad, Sanjeev G Shroff
May 27, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Kirk P Conrad
Jun 30, 2018·Current Opinion in Nephrology and Hypertension·Aline M A de Souza, Crystal A West
Jan 4, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Mark W CunninghamChris Baylis
Mar 22, 2014·American Journal of Physiology. Renal Physiology·Kirk P Conrad, John M Davison
Mar 27, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Mark W CunninghamChris Baylis
Jul 29, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Armin Just
Oct 22, 2016·American Journal of Physiology. Renal Physiology·Crystal A WestChris Baylis
Apr 7, 2020·American Journal of Physiology. Renal Physiology·Hannah R Turbeville, Jennifer M Sasser
Feb 26, 2016·PloS One·Vanessa Meira FerreiraMirian Aparecida Boim
May 4, 2012·American Journal of Physiology. Renal Physiology·Idit F SchwartzDoron Schwartz
May 1, 2015·American Journal of Physiology. Renal Physiology·Crystal A WestChris Baylis
Feb 19, 2021·Biosensors & Bioelectronics·Shuang ZhaoGuixue Wang
Jun 16, 2021·Journal of the American Society of Nephrology : JASN·Jin WeiRuisheng Liu

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