PMID: 2125115Oct 8, 1990Paper

Renal palmitate transport: possible sites for interaction with a plasma membrane fatty acid-binding protein

Molecular and Cellular Biochemistry
M E Trimble

Abstract

In previous studies from this laboratory, a mediated transport system for long chain fatty acids was observed in rat renal basolateral membrane vesicles. Transport was measured in the absence of albumin and indicated the presence of a Na+ independent anion exchange mechanism. The present experiments were done to characterize renal transport of fatty acids derived from fatty acid-albumin complexes. 3H-palmitate uptake by brush border (BBMV) and basolateral membrane vesicles (BLMV) isolated from rat renal cortex was determined using a rapid filtration technique. All incubation media contained 100 microM 3H-palmitate complexed to 100 microM bovine serum albumin. Up to 65% of initially bound fatty acid-albumin complexes were displaceable by washing with solution containing 0.1% albumin. Total palmitate uptake was measured as the remaining non-displaceable radioactivity. In BBMV in low ionic strength (300 mM mannitol) or ionic buffers (100 mM mannitol + 100 mM NaCl or KCl), total palmitate uptake at 15 sec did not differ from equilibrium (60 min) values of 10-11 nmoles/mg protein. Uptake was primarily due to binding. A similar pattern was seen with BLMV in 300 mM mannitol buffer. In BLMV in 100 mM NaCl or KCl buffers, equilibrium up...Continue Reading

Citations

Apr 1, 1997·The Journal of Peptide Research : Official Journal of the American Peptide Society·H VorumB Honoré

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