Renal (pro)renin receptor upregulation in diabetic rats through enhanced angiotensin AT1 receptor and NADPH oxidase activity

Experimental Physiology
Helmy M Siragy, Jiqian Huang

Abstract

Recent studies have demonstrated the presence of the (pro)renin receptor (PRR) in the glomerular mesangium and the subendothelial layer of the renal arteries. We hypothesized that diabetes upregulates PRR expression through enhanced angiotensin subtype 1 (AT1) receptor-NADPH oxidase cascade activity. Using real-time polymerase chain reaction, Western blot analysis and immunostaining, we studied renal localization of the PRR in the streptozotocin-induced diabetic rat model and in response to 1 week of treatment with the AT1 receptor blocker valsartan (10 mg kg(-1) day(-1)), the angiotensin AT2 receptor blocker PD123319 (0.5 mg kg(-1) day(-1)) or the NADPH oxidase inhibitor diphenylene iodonium (DPI; 0.5 mg kg(-1) day(-1)) 6 weeks post-induction of diabetes. Both PRR mRNA and protein were expressed constitutively in the kidneys of normal rat renal cortex and medulla, mainly in glomerular mesangium, proximal, distal and collecting tubules. Compared with normal rats (100%), diabetic rats demonstrated an increase in renal PRR mRNA (184%), protein (228%) and immunostaining. Valsartan and DPI prevented the increase in the PRR mRNA (106 and 126%, respectively), protein (97 and 140%, respectively) and immunostaining that was seen in the...Continue Reading

References

Feb 4, 1999·Regulatory Peptides·W G Thomas
Feb 10, 1999·The American Journal of Physiology·J R IngelfingerS S Tang
Aug 13, 1999·The American Journal of Physiology·J D ImigL G Navar
Feb 24, 2001·American Journal of Physiology. Renal Physiology·G J WehbiK D Burns
Sep 22, 2001·The New England Journal of Medicine·E J LewisUNKNOWN Collaborative Study Group
Sep 22, 2001·The New England Journal of Medicine·B M BrennerUNKNOWN RENAAL Study Investigators
Dec 12, 2001·American Journal of Physiology. Renal Physiology·Lisa M Harrison-BernardL Gabriel Navar
Jan 12, 2002·Kidney International·Maristela Lika OnozatoChristopher S Wilcox
Nov 16, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Chhinder P SodhiAtul Sahai
Aug 2, 2003·Trends in Endocrinology and Metabolism : TEM·Robert M Carey, Helmy M Siragy
Nov 18, 2003·Acta Physiologica Scandinavica·C S Wilcox
Sep 28, 2005·Hypertension·A H Jan Danser, Jaap Deinum
Dec 13, 2005·Current Opinion in Nephrology and Hypertension·Helmy M Siragy
Mar 10, 2006·Journal of the Renin-angiotensin-aldosterone System : JRAAS·A H Jan Danser, Jaap Deinum
Jun 2, 2006·Journal of the American Society of Nephrology : JASN·Atsuhiro IchiharaTadashi Inagami
Feb 13, 2007·Current Opinion in Nephrology and Hypertension·Geneviève Nguyen
May 15, 2007·Journal of the American Society of Nephrology : JASN·Yuki KaneshiroHiroshi Itoh
Jul 20, 2007·American Journal of Physiology. Renal Physiology·Hiroyuki KoboriL Gabriel Navar

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Citations

Jul 16, 2009·Current Hypertension Reports·Helmy M Siragy
Nov 7, 2009·Hypertension Research : Official Journal of the Japanese Society of Hypertension·A H Jan Danser
Dec 17, 2009·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Heiko Funke-KaiserThomas Unger
Dec 19, 2009·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Atsuhiro IchiharaHiroshi Itoh
Feb 3, 2011·Cardiology in Review·Howard S WeintraubArthur Schwartzbard
Nov 26, 2010·Current Opinion in Nephrology and Hypertension·Jennifer L Wilkinson-BerkaKatrina J Binger
Sep 23, 2009·Clinical and Experimental Pharmacology & Physiology·Luis C MatavelliHelmy M Siragy
Jan 20, 2010·Clinical and Experimental Pharmacology & Physiology·Yuki MizuguchiHiroshi Itoh
Jan 10, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Kelly PutnamLisa A Cassis
Aug 5, 2011·International Journal of Biological Sciences·Jingmei DongWeihua Xiao
Mar 12, 2013·International Journal of Hypertension·Takuya Kishi, Yoshitaka Hirooka
Apr 9, 2013·Clinical and Experimental Nephrology·Kazu HamadaYoshio Terada
May 28, 2010·Kidney International·Gabin SihnMichael Bader
Oct 1, 2015·Journal of Diabetes Research·Songyan WangLining Miao
Apr 13, 2012·Clinical Science·Wendy W Batenburg, A H Jan Danser
Nov 23, 2010·Clinical Science·Genevieve Nguyen
Apr 24, 2012·Cellular Signalling·Gowraganahalli JagadeeshNorman Stockbridge
Jul 2, 2016·PloS One·Yoshifumi AmariAtsuhiro Ichihara
Oct 15, 2011·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Luciana G PereiraMirian A Boim
Mar 15, 2012·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Orly VardenyUNKNOWN ALLAY Investigators
May 5, 2010·Nature Reviews. Nephrology·Anne-Emilie Declèves, Kumar Sharma
May 27, 2016·PloS One·Naro OhashiHideo Yasuda
Mar 9, 2017·Journal of the American Society of Nephrology : JASN·Zhen LiYouhua Liu
Nov 21, 2012·Nature Reviews. Nephrology·Bruno Sevá PessôaA H Jan Danser
Jul 31, 2014·American Journal of Physiology. Endocrinology and Metabolism·Arata FukushimaHiroyuki Tsutsui
May 2, 2008·Experimental Physiology·Mohan K Raizada, Julian F R Paton
May 23, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Wencheng LiYumei Feng
Nov 28, 2009·Annals of Medicine·Pim van der HarstDirk J van Veldhuisen
Jul 5, 2011·Journal of Cellular and Molecular Medicine·Hasan MahmudRudolf A de Boer

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