Renal-Protective Effects of the Peroxisome Proliferator-Activated Receptor-γ Agonist Pioglitazone in ob/ob Mice

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Ying LiGuangping Li

Abstract

BACKGROUND This study investigated the therapeutic effects of the peroxisome proliferator-activated receptor-γ (PPARγ) agonist pioglitazone in ob/ob mice with obesity-related glomerulopathy (ORG). MATERIAL AND METHODS A total of 24 mice were divided into 3 groups: wild-type C57BL/6 mice (n=8), ob/ob mice (n=8), and ob/ob mice receiving pioglitazone treatment (n=8). Body mass, blood glucose, serum adiponectin (ADP), and urine microalbumin (mALB) levels were determined. Renal histology was examined using light and electron microscopy. Wilms tumor 1 (WT1), Zonula occludens-1 (ZO-1), AMP activated protein kinase (AMPK), and NADPH oxidase-4 (NOX-4) expression were evaluated by immunohistochemistry and Western blot. RESULTS Serum ADP did not alter between weeks 0 and 12 in the control group, while the ob/ob mice showed a time-dependent decrease that was prevented by pioglitazone. Urinary mALB did not alter between week 0 and 12 in the control group, but was higher in week 0 and week 12 in the ob/ob group. Pioglitazone prevented the rise in urinary mALB in week 12. Histology revealed glomerulomegaly, mesangial proliferation, focal segmental glomerulosclerosis, and foot processes fusion in the ob/ob group, which were ameliorated by pio...Continue Reading

Citations

Oct 31, 2020·International Journal of Molecular Sciences·Florian JuszczakAnne-Emilie Declèves
Apr 6, 2021·Aging and Disease·Yu-Hsiang Chou, Yung-Ming Chen
May 26, 2020·Cardiovascular & Hematological Agents in Medicinal Chemistry·Roya Kazemi, Seyed J Hosseinimehr

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
light microscopy
protein assay
electrophoresis

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