Renal proximal tubular acidification. Role of brush-border and cytoplasmic carbonic anhydrase

Acta Physiologica Scandinavica
B KarlmarkP J Wistrand


Carbonic anhydrase is found in the cytoplasm and brush border membranes of renal proximal tubular cells. Both the soluble and the membrane-bound enzyme have been assigned roles for the secretion of hydrogen ions into the tubular fluid and hence also for the reabsorption of bicarbonate. Attempts were made to differentiate between the roles of these enzymes for the rate of proximal tubular acidification. Proximal tubules of rats were instilled and perfused with bicarbonate solutions containing carbonic anhydrase inhibitors, especially designed to be impermeable to cell membranes. The acidification rate was measured with an antimony micro-electrode system--the only instantly responding micro-pH electrode. The membrane impermeable inhibitors had no effect on this rate in contrast to acetazolamide, which markedly inhibited the acidification rate when administered intraluminally. It is therefore concluded that the cytoplasmic carbonic anhydrase is the important enzyme for the proximal tubular acidification rate, and hence the rate of bicarbonate reabsorption. The function of the brush border enzyme remains an outstanding problem.


Aug 1, 1978·Kidney International·C R CaflischN W CARTER
Feb 1, 1979·Acta Physiologica Scandinavica·M Sohtell
Apr 12, 1977·Biochimica Et Biophysica Acta·P J Wistrand, T Wåhlstrand
Aug 29, 1977·Pflügers Archiv : European journal of physiology·P J Wistrand, R Kinne
Sep 1, 1975·European Journal of Biochemistry·J WistrandT Wåhlstrand
Jan 2, 1975·Biochemical and Biophysical Research Communications·G VanquelinA D Strosberg
Apr 1, 1971·Acta Physiologica Scandinavica·G Lönnerholm
Feb 28, 1972·Biochimica Et Biophysica Acta·R P Poincelot
May 1, 1973·Analytical Biochemistry·B Karlmark, M Sohtell
Apr 1, 1968·The American Journal of Physiology·F L Vieira, G Malnic
Jun 1, 1971·The American Journal of Physiology·G Malnic, M de Mello-Aires
Feb 1, 1968·The American Journal of Physiology·B A Bernstein, J R CLAPP
Oct 1, 1963·The American Journal of Physiology·J R CLAPPR W Berliner
Feb 1, 1965·The Journal of Clinical Investigation·F C RECTORD W SELDIN
Jan 1, 1968·The Journal of Clinical Investigation·C M BennettR W Berliner


Jul 1, 1981·Medical & Biological Engineering & Computing·M Markdahl-Bjarme, G Edwall
Nov 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·B Riehl, W R Schlue
Oct 1, 1994·Neuroscience·I I Grichtchenko, Mitchell Chesler
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D BrownW S Sly
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J C Chen, Mitchell Chesler
Dec 14, 2012·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Syed K HaqueDaniel Batlle
Feb 1, 1979·Acta Physiologica Scandinavica·M Sohtell
Mar 1, 1989·Kidney International·P J Wistrand, K G Knuuttila
Nov 1, 1982·The Anatomical Record·D C DobyanR E Bulger
Jan 1, 1980·Annals of the New York Academy of Sciences·B Karlmark
Nov 6, 2014·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Daniel KittererJoerg Latus
Jun 28, 2019·International Journal of Molecular Sciences·Yuri Dornelles ZebralAdalto Bianchini
Jan 1, 1984·Annals of the New York Academy of Sciences·P J Wistrand
Sep 25, 2003·Physiological Reviews·Mitchell Chesler
Jun 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·E R SwensonC Tu

Related Concepts

Acetazolamide Sodium, (Sterile)
Carbonic Acid Ions
Carbonate Dehydratase
Carbonic Anhydrase Inhibitors
Plasma Membrane
Hydrogen-Ion Concentration
Kidney Tubules, Proximal

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.