Renal proximal tubular cell growth and differentiation are differentially modulated by renotropic growth factors and tyrosine kinase inhibitors

European Journal of Clinical Investigation
F ErnstP M Jehle

Abstract

The renotropic growth factors (GFs), hepatocyte GF (HGF), epidermal GF (EGF), and insulin-like GF-I (IGF-I) accelerate renal regeneration in animal models after toxic or ischemic injury. These GFs initiate their biological effects on renal tubular cells by interaction with specific transmembrane receptor tyrosine kinases. In the proximal tubular cell line PT-1, the biological effects of HGF, EGF, and IGF-I and the growth-inhibitory effects of different tyrosine kinase inhibitors (TKIs) were investigated. Receptor binding and tyrosine kinase phosphorylation were determined by ligand binding studies and Western blot analysis. HGF, EGF, and IGF-I bound with nanomolar affinity to their specific cell membrane receptor tyrosine kinases. In contrast to EGF or IGF-I, HGF induced a variety of cell morphological changes, including cell scattering, formation of tubular structures, and expression of long microvilli on the apical cell membrane. HGF was a 10-fold more potent and more effective growth promoter than EGF or IGF-I. Among the TKIs tested, the mitogenic effect of HGF could be more specifically inhibited by emodin and tyrphostin, that of EGF by methyl-2,5-dihydroxycinnamate, lavendustin A, and genistein, and that of IGF-I by geldan...Continue Reading

References

Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S B MillerM R Hammerman
May 1, 1992·Journal of Natural Products·H JayasuriyaC J Chang
Apr 20, 1990·Cell·A Ullrich, J Schlessinger
Sep 15, 1989·Biochemical and Biophysical Research Communications·Y UeharaS Mizuno
Jan 1, 1993·Annual Review of Biochemistry·W J FantlL T Williams
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·K KawaidaT Nakamura
Jun 21, 1996·International Journal of Cancer. Journal International Du Cancer·P G NataliM F Di Renzo
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·H TakayamaG Merlino
Mar 20, 1997·The New England Journal of Medicine·H D Humes
Aug 1, 1997·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·S Wang, R Hirschberg
Jan 13, 1998·Biochimica Et Biophysica Acta·A BardelliP M Comoglio
Jun 26, 1998·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·S StrackeP M Jehle
Oct 3, 1998·Intensive Care Medicine·G VivinoA Gasparetto
Oct 31, 1998·Nihon Ronen Igakkai zasshi. Japanese journal of geriatrics·S Okuda
Jan 5, 2000·The Journal of Clinical Investigation·B J Druker, N B Lydon
Apr 12, 2000·Kidney International·G A VargasP M Jehle

❮ Previous
Next ❯

Citations

Oct 31, 2007·Apoptosis : an International Journal on Programmed Cell Death·H ServaisM-P Mingeot-Leclercq
Oct 9, 2003·Kidney International·Prabal K ChatterjeeChristoph Thiemermann
Oct 3, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Sven KroeningMargarete Goppelt-Struebe
Aug 19, 2007·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·P TuncyurekS Stracke
Nov 6, 2007·Biological & Pharmaceutical Bulletin·Jing-Dong XuJin-Xia Zhu
Oct 19, 2007·Journal of the American Society of Nephrology : JASN·Barbara ImbertiGiuseppe Remuzzi
Sep 9, 2006·Kidney & Blood Pressure Research·Patrick C Baer, Helmut Geiger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved