Renal tubule-specific transcription and chromosomal localization of rat thiazide-sensitive Na-Cl cotransporter gene

The Journal of Biological Chemistry
Y TaniyamaK Takeuchi

Abstract

The molecular mechanism underlying the renal expression localization of the thiazide-sensitive Na-Cl cotransporter (TSC) gene was studied. The TSC gene was localized to chromosome 19p12-14. In cultured cells, tissue-specific transcription activity of the 5'-flanking region of the rat rTSC gene (5'FL/rTSC) was demonstrated, and the major promoter region was located between position -580 and -141. To further examine the tissue-specific transcription, transgenic rats harboring the 5'FL/rTSC fused upstream of the LacZ gene were generated. Immunohistochemical analysis clearly showed that LacZ gene expression was co-localized to distal convoluted tubules (DCT) with TSC, indicating that the 5'FL/rTSC regulates the renal tubule-specific TSC expression. Because a transcription factor, HFH-3 (hepatocyte nuclear factor-3/folk head homologue-3), had also been localized to DCT, a possible role of the putative cis-acting element (HFH-3/rTSC, -400/-387 position) for HFH-3 binding in the tissue-specific transcription was examined. Deletion and mutation analyses suggested that transcription of the HFH-3/rTSC was actually responsive to HFH-3, and electrophoretic mobility shift assay showed a direct binding of in vitro synthesized HFH-3 to the HF...Continue Reading

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Citations

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Jan 28, 2014·Pflügers Archiv : European journal of physiology·P GaillyO Devuyst
Feb 3, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yuji HigashibataShintaro Nomura
Aug 16, 2002·The Tohoku Journal of Experimental Medicine·Kazuhisa Takeuchi
Apr 7, 2015·Acta Biochimica Et Biophysica Sinica·Linghong WangXiulan Su
Sep 9, 2004·American Journal of Physiology. Renal Physiology·Edna EfratiIsrael Zelikovic
Mar 7, 2008·American Journal of Physiology. Cell Physiology·Lior AdlerIsrael Zelikovic
Jul 14, 2020·American Journal of Physiology. Renal Physiology·Ryan J CorneliusJames A McCormick
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Feb 15, 2007·American Journal of Physiology. Renal Physiology·Masashi SakaiSatoshi Umemura

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