Renewable and tuneable bio-LPG blends derived from amino acids

Biotechnology for Biofuels
Mohamed AmerNigel S Scrutton

Abstract

Microbial biorefinery approaches are beginning to define renewable and sustainable routes to clean-burning and non-fossil fuel-derived gaseous alkanes (known as 'bio-LPG'). The most promising strategies have used a terminal fatty acid photodecarboxylase, enabling light-driven propane production from externally fed waste butyric acid. Use of Halomonas (a robust extremophile microbial chassis) with these pathways has enabled bio-LPG production under non-sterile conditions and using waste biomass as the carbon source. Here, we describe new engineering approaches to produce next-generation pathways that use amino acids as fuel precursors for bio-LPG production (propane, butane and isobutane blends). Multiple pathways from the amino acids valine, leucine and isoleucine were designed in E. coli for the production of propane, isobutane and butane, respectively. A branched-chain keto acid decarboxylase-dependent pathway utilising fatty acid photodecarboxylase was the most effective route, generating higher alkane gas titres over alternative routes requiring coenzyme A and/or aldehyde deformylating oxygenase. Isobutane was the major gas produced in standard (mixed amino acid) medium, however valine supplementation led to primarily propa...Continue Reading

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Citations

Dec 3, 2020·Synthetic Biology·Duangthip TrisriviratNigel S Scrutton
Nov 15, 2020·Microbial Cell Factories·Mohamed AmerNigel S Scrutton
Jun 3, 2021·Microorganisms·Alec BannerNigel S Scrutton

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Methods Mentioned

BETA
deamination
PCR

Software Mentioned

BioRad ImageLab

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