Renin-renin substrate kinetic constants in the plasma of normal and estrogen-treated humans

The Journal of Clinical Endocrinology and Metabolism
W J McDonaldJ W Conn

Abstract

The renin-renin substrate Michaelis constant (Km) and maximal velocity (Vmax) were determined in the plasmas of normal subjects. The mean Km was 0.7 microgram/ml. Under these conditions, the in vitro reaction of renin with physiologic concentrations of renin substrate will proceed at only 70% of its maximal velocity. Following estrogen administration, Km doubled to a value of 1.3 microgram/ml. Vmas increased by 81%. Analysis of the changes induced in the in vitro reaction velocity demonstrated that estrogen-induced acceleration of the renin reaction is dependent upon both an increase in renin substrate concentration as well as an increase in Vmas. The latter appears to be quantitatively more important. These findings suggest the emergence of modifying factors in the renin-renin substrate interaction following estrogen administration.

Citations

Dec 1, 1986·Biochemical Medicine and Metabolic Biology·R C FranksR H Hayashi
Jan 1, 1983·Journal of the American College of Cardiology·E D Frohlich
Jan 1, 1980·Clinical and Experimental Hypertension : CHE·D B Gordon, I N Sachin
Jan 1, 1991·Clinical and Experimental Hypertension. Part A, Theory and Practice·P EggenaM S Golub
Jun 1, 1979·Biochemical Medicine·P EpggenaM P Sambhi
Nov 1, 1981·Clinics in Endocrinology and Metabolism·J M Roberts
Dec 1, 1980·Biochemical Medicine·A B GouldC Swartz
Sep 1, 1991·Journal of Endocrinological Investigation·J E KalinyakA J Perlman
Apr 1, 1988·The American Journal of Physiology·P Eggena, J D Barrett

Related Concepts

Renin Substrate Tetradecapeptide
Angiotensins
Progynon C
Mestranol
Norethindrone, (1 beta)-Isomer
Preprorenin
Estradiol Congeners

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