Renomedullary interstitial cells in culture; the osmolality and oxygen tension influence the extracellular amounts of hyaluronan and cellular expression of CD44

Matrix Biology : Journal of the International Society for Matrix Biology
V GöranssonC Maric

Abstract

Our previous studies have suggested a role for renomedullary interstitial cells (RMICs) and renal medullary hyaluronan (HA) in water homeostasis. In the present study, cultured rat RMICs were used to examine the relationship of osmolality and oxygen tension on the extracellular amount of HA in the culture and to the cellular immunoreactivity to CD44, a HA binding protein. Under isotonic (330 mOsm(.)kg(-1) H(2)O), normoxic (20% O(2)) conditions, supernatant from sub-confluent RMICs contained 120+/-37 pg 10(4) cells(-1) 24 h(-1) of HA. Under hyperosmotic conditions (630 mOsm kg(-1) H(2)O), HA in the supernatant was decreased by 42% and under hypoosmotic conditions (230 mOsm kg(-1) H(2)O) it was doubled. Under hypoxic, iso-osmolar conditions (5% and 1% O(2), 330 mOsm kg(-1) H(2)O) this HA content was decreased by 56 and 48%, respectively, compared with normoxic, iso-osmolal conditions. Expression of CD44 on sub-confluent cells increased with increasing osmolality, as shown by immunostaining and flow cytometric analysis. The increases in CD44 from 330 to 630, 930 and 1230 mOsm kg(-1) H(2)O amounted to 5, 142 and 212%, respectively. Low oxygen tension (5% O(2)) decreased the intensity of CD44 immunofluorescence by 31%. Cell viabilit...Continue Reading

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Citations

Mar 20, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Viktoria GöranssonPeter Hansell
Jun 24, 2010·Current Opinion in Nephrology and Hypertension·Jens Titze, Agnes Machnik
Dec 18, 2007·Acta Physiologica·L RügheimerP Hansell
May 23, 2015·Urolithiasis·Jin-Yi LiTie Zhou
Jul 29, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·Louise RügheimerPeter Hansell
Jul 4, 2002·The Journal of Physiology·Viktoria GöranssonPeter Hansell
Oct 25, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Evgenia KarousouSpyros S Skandalis
Jun 25, 2017·Physiological Reports·Omar Flores-SandovalManuel Rodríguez-Martínez
May 19, 2006·Journal of the American Society of Nephrology : JASN·Carl Friedrich Verkoelen
Jun 11, 2009·Bulletin of Experimental Biology and Medicine·L V ShestopalovaL N Ivanova
Apr 20, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sara StridhPeter Hansell
Dec 14, 2017·International Journal of Molecular Sciences·Sara StridhPeter Hansell
Jul 24, 2004·The Journal of Biological Chemistry·Robert H JenkinsRobert Steadman

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