PMID: 2503830Aug 1, 1989Paper

Reordering of nine exons is necessary to form a functional actin gene in Oxytricha nova

Proceedings of the National Academy of Sciences of the United States of America
A F GreslinJ C Chappell

Abstract

During the development of a macronucleus from a micronucleus after cell mating in hypotrichs all the genes (approximately 20,000) are excised from micronuclear chromosomes as individual small DNA molecules. Telomeres are added to the ends of each gene-sized molecule and each is amplified, mostly by approximately 1000-fold, to yield a transcriptionally active macronucleus. As a part of the study of the excision of genes from chromosomes, we have cloned six fragments of chromosomal DNA from Oxytricha nova, each containing a full copy of an actin gene, for comparison with the structure of the actin-encoding DNA molecule in the macronucleus. All six micronuclear actin clones had the same overall organization as judged by restriction mapping. Two micronuclear actin clones were sequenced. These differ from one another at a few nucleotide positions but both prescribe precisely the same actin polypeptide. Both micronuclear actin genes contain nine exons separated by eight intron-like sequences. The macronuclear gene contains these nine exons without intron-like segments. Assigning the order 1 through 9 to the nine micronuclear exons, the order in the macronucleus is 8-7-1-2-4-3-5-9-6. In the micronuclear actin gene, all nine exons poss...Continue Reading

Citations

May 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D M Prescott
Jan 1, 1992·Developmental Genetics·D M Prescott, A F Greslin
Dec 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Meng-Chao Yao
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·M DuBois, D M Prescott
Jun 11, 1992·Nucleic Acids Research·E D LampertiL Villa-Komaroff
Sep 1, 1994·The Journal of Eukaryotic Microbiology·J L MitchamM K Miller
Nov 1, 1996·The Journal of Eukaryotic Microbiology·D M Prescott, M L DuBois
Nov 1, 1996·The Journal of Eukaryotic Microbiology·M E Jacobs, L A Klobutcher
Dec 29, 1998·The Journal of Eukaryotic Microbiology·D M Prescott
Jul 23, 1999·Annals of the New York Academy of Sciences·J A Shapiro
Aug 2, 2011·Annual Review of Genomics and Human Genetics·Mariusz NowackiLaura F Landweber
Jan 1, 1992·Genetica·J A Shapiro
Dec 1, 1990·Experientia·R Cattaneo
Aug 31, 1990·European Journal of Protistology·G Steinbrück
Dec 1, 2005·Gene·Wei-Jen ChangLaura F Landweber
Nov 16, 2005·Annual Review of Genetics·Meng-Chao Yao, Ju-Lan Chao
Jul 27, 2002·Annual Review of Microbiology·Carolyn L Jahn, Lawrence A Klobutcher
Jan 1, 1995·Artificial Life·T Ikegami, T Hashimoto
Jan 11, 2008·Nature·Meng-Chao Yao
Aug 12, 2010·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Laura A Katz, Alexandra M Kovner
Sep 16, 2005·Molecular Biology and Evolution·Shiuhyang KuoLaura F Landweber
May 29, 2020·Cellular and Molecular Life Sciences : CMLS·Iwona RzeszutekMariusz Nowacki
Jan 1, 1997·Molecular and Cellular Biology·M L DuBois, D M Prescott
Jun 25, 2015·Microbiology Spectrum·V Talya Yerlici, Laura F Landweber
Jun 1, 1994·Microbiological Reviews·D M Prescott

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