Reorganization of a synthetic microbial consortium for one-step vitamin C fermentation

Microbial Cell Factories
En-Xu WangYing-Jin Yuan

Abstract

In the industry, the conventional two-step fermentation method was used to produce 2-keto-L-gulonic acid (2-KGA), the precursor of vitamin C, by three strains, namely, Gluconobacter oxydans, Bacillus spp. and Ketogulonicigenium vulgare. Despite its high production efficiency, the long incubation period and an additional second sterilization process inhibit the further development. Therefore, we aimed to reorganize a synthetic consortium of G. oxydans and K. vulgare for one-step fermentation of 2-KGA and enhance the symbiotic interaction between microorganisms to perform better. During the fermentation, competition for sorbose of G. oxydans arose when co-cultured with K. vulgare. In this study, the competition between the two microbes was alleviated and their mutualism was enhanced by deleting genes involved in sorbose metabolism of G. oxydans. In the engineered synthetic consortium (H6 + Kv), the yield of 2-KGA (mol/mol) against D-sorbitol reached 89.7 % within 36 h, increased by 29.6 %. Furthermore, metabolomic analysis was used to verify the enhancement of the symbiotic relationship and to provide us potential strategies for improving the synthetic consortium. Additionally, a significant redistribution of metabolism occurred ...Continue Reading

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Citations

Jun 1, 2016·Metabolic Engineering·Haoran Zhang, Xiaonan Wang
Dec 14, 2017·Critical Reviews in Biotechnology·Gloria PadmaperumaSeetharaman Vaidyanathan
Jul 7, 2019·Journal of Industrial Microbiology & Biotechnology·Shun Che, Yujie Men
Oct 18, 2018·BMC Genomics·Angela KranzTino Polen
Sep 6, 2020·Evolution; International Journal of Organic Evolution·Chang-Yu ChangAlvaro Sanchez
Jan 7, 2018·BMC Genomics·Angela KranzTino Polen
Apr 23, 2019·Chemical Engineering & Technology·Vasileios PothakosTodd Anderson
Sep 26, 2020·Applied Microbiology and Biotechnology·Philipp Moritz FrickeTino Polen
Jan 30, 2019·Scientific Reports·Tae-Su KimJung-Kul Lee
Dec 22, 2019·Biotechnology Advances·Xiujuan QianKatrin Ochsenreither
Feb 1, 2021·Current Opinion in Biotechnology·Martijn DienderDiana Z Sousa
Jun 29, 2021·Critical Reviews in Biochemistry and Molecular Biology·Matthew WongMattheos Koffas
Aug 28, 2021·Applied Microbiology and Biotechnology·Philipp Moritz FrickeTino Polen
Apr 4, 2019·Metabolic Engineering·Volker F Wendisch

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Methods Mentioned

BETA
PCR
without genetic modification
PCA

Software Mentioned

MetaboAnalyst

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