Reorientation of large DNA molecules in concentrated polyacrylamide solution during crossed-field electrophoresis

Electrophoresis
H OanaK Yoshikawa

Abstract

Recently, we found that, in concentrated neutral solutions, DNA molecules migrate in linear conformation under steady electric field. In this paper, we report the conformational change of DNA during 120 degree crossed-field electrophoresis in the same polymer solution. We found that, in concentrated polyacrylamide solutions, the reorientation process of DNAs becomes simple: the DNA goes back along the previous track and the reorientation time is longer for larger DNA. Such a backtrack motion has been thought to be an essential motion for the separation of DNA fragments in pulsed field gel electrophoresis. We expect that this phenomenon is useful for a more efficient separation technique of large DNAs than the current pulsed field gel electrophoresis.

References

May 1, 1992·Quarterly Reviews of Biophysics·B H Zimm, S D Levene
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G Chu
May 1, 1991·Quarterly Reviews of Biophysics·B NordénB Akerman
Jun 1, 1991·Molecular and Cellular Biology·K Gunderson, G Chu
Aug 11, 1987·Nucleic Acids Research·E M SouthernD S Fletcher
Jun 1, 1996·Electrophoresis·T A DukeS S Chan
Sep 14, 1987·Physical Review Letters·J M Deutsch
Jan 27, 1992·Physical Review Letters·T A Duke, J L Viovy

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Citations

Aug 27, 1998·Electrophoresis·G W SlaterG Drouin
May 31, 2001·Journal of Chromatography. a·Z KabátekB Gas
Oct 13, 2006·Biophysical Chemistry·M UedaK Yoshikawa
Nov 18, 1998·Current Opinion in Chemical Biology·T Duke
Dec 23, 2008·Journal of Chromatography. a·Nancy C Stellwagen, Earle Stellwagen
Dec 31, 2003·Analytical Chemistry·Ming-Feng HuangHuan-Tsung Chang

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