PMID: 7018583May 14, 1981Paper

Reorientations of coenzyme in aspartate transaminase studied on single crystals of the enzyme by polarized-light spectrophotometry

Biochimica Et Biophysica Acta
V L MakarovY M Torchinsky

Abstract

Investigation of polarized-light absorption spectra of single crystals of cytosolic aspartate transaminase (L-aspartate:2-oxoglutarate aminotransferase, EC 2.6.1.1) from chicken heart has revealed that the coenzyme's absorption bands at 430 and 360 nm are polarized in opposite directions, both in crystals of the free enzyme and in its complex with a quasi-substrate, 2-methylaspartate. The opposite signs of polarization of the 430 and 360 nm bands of the free enzyme indicate different orientation of the pyridine ring of pyridoxal 5'-phosphate in the protonated and non-protonated forms of the "internal" coenzyme-lysine aldimine. These data suggest that reorientation of the coenzyme ring occurs mainly in the first step of the catalytic reaction, associated with proton transfer from the NH+3 group of amino acid substrate to the coenzyme-lysine aldimine. Absorption bands at 333 and 430 nm are seen in the spectra upon soaking the crystals in solutions containing aspartate, glutamate or cysteinesulfinate. Both bands are polarized in the same direction as is the 430 nm absorption bands of the protonated internal aldimine. Soaking the crystals in solutions containing 2-oxoglutarate, glutarate or maleate reverses the sign of polarization...Continue Reading

References

Nov 5, 1978·Journal of Molecular Biology·V V BorisovA E Braunstein
Apr 30, 1966·Nature·M Y Karpeisky, V I Ivanov
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G C FordJ N Jansonius

❮ Previous
Next ❯

Citations

Dec 24, 1997·Molecular Biotechnology·D B VolkinC R Middaugh
Apr 10, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Anna Pastore, Fiorella Piemonte
Jul 21, 2010·Journal of Molecular Biology·Andrea HallP Andrew Karplus
Apr 24, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lucía TurellBeatriz Alvarez
Jun 10, 2008·British Journal of Haematology·Nagaraj ManickamDavid W Essex
Jul 12, 2008·Journal of Peptide Science : an Official Publication of the European Peptide Society·Takahide KounoKeiichi Kawano
Mar 29, 2011·The FEBS Journal·Elisabetta PasseraAndrea Mozzarelli
Oct 10, 2013·Mass Spectrometry Reviews·Cristina M Furdui, Leslie B Poole
Nov 17, 1986·Biochimica Et Biophysica Acta·N M Scherer, D W Deamer
May 15, 1985·Archives of Biochemistry and Biophysics·R J Scott-Ennis, E A Noltmann
Feb 13, 2007·Free Radical Biology & Medicine·Isabella Dalle-DonneGiancarlo Aldini
Jan 1, 2007·Environmental Toxicology and Pharmacology·T Wayne SchultzTyler B Pilkington
Dec 1, 1988·Archives of Biochemistry and Biophysics·G A MooreP J O'Brien
Feb 23, 1999·Pharmaceutical Development and Technology·W P YeF S Du
Jul 24, 1998·Archives of Biochemistry and Biophysics·P Di SimplicioR Rossi
Jun 12, 2013·Free Radical Biology & Medicine·Lucía TurellBeatriz Alvarez
Jan 12, 1999·Archives of Biochemistry and Biophysics·H BjörkbackaC Forsman
Jan 13, 2015·Archives of Biochemistry and Biophysics·Hemanth P K Sudhani, Joaquín Moreno
Mar 1, 1997·Archives of Biochemistry and Biophysics·H ZhangW A Pryor
Mar 1, 1997·Journal of Pharmaceutical Sciences·V WindischM Vuilhorgne
Jul 5, 1997·Biotechnology and Bioengineering·S T Kelly, A L Zydney
Oct 5, 1990·European Journal of Biochemistry·L N ZakomirdinaY M Torchinsky
Jul 1, 1991·Bioconjugate Chemistry·R A BednarK Shan
Nov 1, 1989·Biotechnology and Bioengineering·E BarzanaA M Klibanov
Jan 1, 1985·Biochemistry·P C Harrington, R G Wilkins
Mar 31, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·L TurellB Alvarez
Jan 8, 1991·Biochemistry·R BalhornB Watkins
Jan 1, 1986·Journal of Free Radicals in Biology & Medicine·N M Scherer, D W Deamer
Oct 25, 2006·Amino Acids·S CarballalR Radi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.