PMID: 9429249Jan 16, 1998Paper

REP-PCR analysis of Pasteurella multocida isolates that cause haemorrhagic septicaemia

Research in Veterinary Science
K M TownsendJ M Papadimitriou

Abstract

Amplification of multiple P multocida genomic DNA fragments by outwardly-directed primers based on the repetitive extragenic palindromic (REP) consensus sequence, generated complex profiles in a PCR-based fingerprinting method known as REP-PCR. Polymorphisms within REP-PCR profiles were used to characterise 38 isolates of P multocida. The high degree of homogeneity observed among haemorrhagic septicaemia (HS) strains of serotype B and E provided evidence of a disease-associated REP profile that may serve as a novel method for the identification of HS strains regardless of serotype. REP-PCR profiles of other P multocida serotypes were highly variable, illustrating the potential of this technique for the molecular fingerprinting of fowl cholera or atrophic rhinitis isolates. A specific amplified REP fragment was isolated and used to probe membrane-bound digested P multocida genomic DNA. Hybridisation patterns not only distinguished HS-causing isolates from non-HS P multocida, but also demonstrated a degree of relatedness between HS and HS-like strains.

References

Jan 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P R GeorghiouJ R Lupski

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Citations

Nov 10, 2011·Tropical Animal Health and Production·Edgardo Soriano-VargasErasmo Negrete-Abascal
Dec 16, 1998·Veterinary Microbiology·K M TownsendA J Frost
Mar 4, 2000·Veterinary Microbiology·M L HuntK M Townsend
Jun 17, 2011·Animal Health Research Reviews·S B ShivachandraA A Kumar
Mar 16, 2006·Avian Pathology : Journal of the W.V.P.A·S B ShivachandraS K Srivastava
Aug 1, 2002·Journal of Clinical Microbiology·Alongkorn AmonsinVivek Kapur
Jul 5, 2013·Clinical Microbiology Reviews·Brenda A Wilson, Mengfei Ho
Aug 7, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yanhong WangXiufan Liu
Jul 7, 2005·Microbial Pathogenesis·Fred M TatumRobert E Briggs
Jul 6, 2004·Journal of Microbiological Methods·Dongyou LiuFrank W Austin
Jul 17, 2007·Veterinary Microbiology·Zoran JaglicPavel Alexa
Jun 1, 2007·Comparative Immunology, Microbiology and Infectious Diseases·S B ShivachandraP Chaudhuri
Aug 3, 2007·Comparative Immunology, Microbiology and Infectious Diseases·D R KaloreyA G Bhandarkar
Nov 11, 2009·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Anina B J StahelBozena M Korczak

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