Repair of acetyl-aminofluorene modified pBR322 DNA in Xenopus laevis oocytes and eggs; effect of diadenosine tetraphosphate

Biochimie
G OrfanoudakisP Remy

Abstract

Using Xenopus laevis oocytes and unfertilized eggs, we have developed a system which allows the study of DNA repair upon microinjection of pBR 322 DNA which has been previously modified in vitro by N-acetyl-aminofluorene, under controlled conditions. In unfertilized eggs, an efficient repair of pBR-18AAF DNA takes place, leading to a restoration of the transforming activity of the plasmid DNA towards Escherichia coli. The repaired DNA is even efficiently replicated, the egg being "activated" by the microinjection. In the oocyte, a partial repair is observed as shown by the incorporation of labelled dCTP in the modified plasmid DNA, even in the presence of aphidicolin, an inhibitor of DNA polymerase alpha. However, the repair appears to be very limited, since it does not restore the transforming activity of the modified plasmid DNA. This inefficient repair in the oocyte may be due to the rapid packaging of foreign DNA into a minichromosome and/or to a very low level of DNA polymerase beta. This system was used to study the effect of diadenosine tetraphosphate (Ap4A) on DNA repair. Ap4A seems not to interfere with repair processes in the oocyte, but significantly inhibits the replication following the repair of AAF-modified plasm...Continue Reading

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jul 1, 1979·European Journal of Biochemistry·M OguroS Ikegami
Mar 1, 1975·Developmental Biology·C C Ford, H R Woodland
Dec 1, 1987·Molecular and Cellular Biology·R J LegerskiD A Wright
Jul 1, 1988·Experimental Cell Research·G GilsonP Remy
Feb 28, 1988·Biochimica Et Biophysica Acta·S WilsonS Widen
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J C Baker, M K Jacobson
Jul 1, 1987·Molecular and Cellular Biology·G OrfanoudakisP Remy
Jan 1, 1985·Annual Review of Biochemistry·K Ueda, O Hayaishi
Aug 16, 1984·Biochemical and Biophysical Research Communications·H Juarez-SalinasM K Jacobson
Jan 1, 1983·Methods in Enzymology·J B Gurdon, M P Wickens
May 1, 1984·Cell·M Ryoji, A Worcel
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D Carroll
Feb 7, 1980·Nature·B W DurkaczS Shall
Apr 15, 1981·Biochemical and Biophysical Research Communications·Y TanakaK Yoshihara
Jan 1, 1983·Molecular and Cellular Biology·J E Mertz, T J Miller
May 1, 1984·Cell·G C GlikinA Worcel
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Nov 5, 1983·Journal of Molecular Biology·G Gargiulo, A Worcel
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A Sugino, K Nakayama
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R CorteseD Melton
Jan 1, 1981·Annual Review of Genetics·J B Gurdon, D A Melton
Jan 1, 1981·Annual Review of Genetics·B A Kunz, R H Haynes
Dec 1, 1982·Molecular and Cellular Biology·J E Mertz

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Citations

Sep 29, 2000·Pharmacology & Therapeutics·A G McLennan
Jan 2, 2003·DNA Repair·Thierry Nouspikel, Philip C Hanawalt
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·Y H ChenD F Bogenhagen

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