PMID: 9443981Mar 14, 1998Paper

Repair of degraded duplex DNA from prehistoric samples using Escherichia coli DNA polymerase I and T4 DNA ligase

Nucleic Acids Research
C M PuschM Scholz

Abstract

The most notable feature of DNA extracted from prehistoric material is that it is of poor quality. Amplification of PCR products from such DNA is consequently an exception. Here we present a simple method for the repair of degraded duplex DNA using the enzymes Escherichia coli DNA polymerase I and T4 DNA ligase. Adjacent sequences separated by nicks do not split up into intact strands during the denaturation step of PCR. Thus the target DNA is refractory to amplification. The proposed repair of nicked, fragmented ancient DNA results in an increase of amplification efficiency, such that the correct base order of the respective nuclear DNA segment can be obtained.

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Citations

Mar 8, 2005·Mutation Research·David MitchellAnders Hansen
Dec 21, 2004·Nature Reviews. Microbiology·Michel Drancourt, Didier Raoult
Nov 5, 2013·TheScientificWorldJournal·Stefania Del GaudioMarilena Cipollaro
Oct 18, 2011·Forensic Science International. Genetics·Toni M DiegoliTodd W Bille
Mar 11, 2010·Forensic Science International. Genetics·Reza AlaeddiniAli Abbas
Aug 16, 2015·Forensic Science International. Genetics·Jessica Rothe, Marion Nagy
Sep 25, 2004·Journal of Cellular Physiology·M CipollaroG Di Bernardo
Sep 1, 2009·Molecular Ecology Resources·Albano Beja-PereiraGordon Luikart
Jun 7, 2005·Forensic Science International : Synergy·François-Xavier RicautBertrand Ludes
Jun 17, 2011·BMC Research Notes·Till TöpferElisabeth Haring
Aug 28, 2012·Journal of Archaeological Science·Jennifer D KurushimaLeslie A Lyons
Jul 6, 2014·Forensic Science International. Genetics·James M RobertsonKelly A Meiklejohn
Dec 19, 2002·American Journal of Human Genetics·M Thomas P GilbertAlan Cooper
Sep 1, 2017·International Journal of Legal Medicine·Yuxuan LiuDaixin Huang
Aug 22, 2002·Journal of Agricultural and Food Chemistry·Andree LeRoyAnn M Hirsch

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