Repair of DNA-polypeptide crosslinks by human excision nuclease

Proceedings of the National Academy of Sciences of the United States of America
Joyce T Reardon, A Sancar

Abstract

DNA-protein crosslinks are relatively common DNA lesions that form during the physiological processing of DNA by replication and recombination proteins, by side reactions of base excision repair enzymes, and by cellular exposure to bifunctional DNA-damaging agents such as platinum compounds. The mechanism by which pathological DNA-protein crosslinks are repaired in humans is not known. In this study, we investigated the mechanism of recognition and repair of protein-DNA and oligopeptide-DNA crosslinks by the human excision nuclease. Under our assay conditions, the human nucleotide excision repair system did not remove a 16-kDa protein crosslinked to DNA at a detectable level. However, 4- and 12-aa-long oligopeptides crosslinked to the DNA backbone were recognized by some of the damage recognition factors of the human excision nuclease with moderate selectivity and were excised from DNA at relatively efficient rates. Our data suggest that, if coupled with proteolytic degradation of the crosslinked protein, the human excision nuclease may be the major enzyme system for eliminating protein-DNA crosslinks from the genome.

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J C HuangA Sancar
Feb 10, 1995·The Journal of Biological Chemistry·D MuA Sancar
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·J C HuangA Sancar
Oct 1, 1995·Trends in Biochemical Sciences·D B Zamble, S J Lippard
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·S W YangH A Nash
Nov 20, 1997·The Journal of Biological Chemistry·D MuA Sancar
Jan 24, 2002·The Journal of Biological Chemistry·Michael S DeMottBruce Demple
Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Irina G MinkoR Stephen Lloyd
Jul 26, 2002·Molecular and Cellular Biology·Joyce T Reardon, Aziz Sancar
Dec 6, 2003·Cell Cycle·Leonie M Quinn, Helena Richardson
Jun 11, 2004·Annual Review of Biochemistry·Aziz SancarStuart Linn
Feb 23, 2005·Biochemistry·Irina G MinkoR Stephen Lloyd
Mar 30, 2005·Mutation Research·Sharon BarkerDavid Murray
Aug 13, 2005·Progress in Nucleic Acid Research and Molecular Biology·Joyce T Reardon, Aziz Sancar
Sep 6, 2005·The Journal of Biological Chemistry·Heidrun InterthalJames J Champoux

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Citations

Aug 21, 2008·Chemical Research in Toxicology·Aley G KalapilaAnthony E Pegg
Dec 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jonathan T SczepanskiMarc M Greenberg
May 7, 2010·The Journal of Biological Chemistry·Anuradha KumariAmanda K McCullough
Mar 3, 2007·Nucleic Acids Research·Katerina ChválováJana Kaspárková
Nov 11, 2008·Nucleic Acids Research·Laura A ChristensenKaren M Vasquez
Mar 16, 2007·PLoS Biology·Olivier MaillardHanspeter Naegeli
Oct 30, 2015·Current Opinion in Cell Biology·Julien P Duxin, Johannes C Walter
Oct 27, 2007·Trends in Biochemical Sciences·Olivier MaillardHanspeter Naegeli
Sep 18, 2007·Mutation Research·Irina G MinkoR Stephen Lloyd
Jul 18, 2009·The EMBO Journal·Ulrike CamenischHanspeter Naegeli
Oct 11, 2014·Cell·Julien P DuxinJohannes C Walter
Mar 4, 2015·DNA Repair·Rachel KrasichKenneth N Kreuzer
Dec 28, 2010·Mutation Research·Hiroshi IdeAmir M H Salem
Nov 14, 2014·The Journal of Biological Chemistry·Susith WickramaratneNatalia Y Tretyakova
Jan 19, 2019·Current Protocols in Nucleic Acid Chemistry·Pratibha P GhodkeF Peter Guengerich
Mar 21, 2008·Chembiochem : a European Journal of Chemical Biology·Hauh-Jyun Candy ChenSiou-Siou Yang
May 18, 2007·The Journal of Biological Chemistry·David J BakerTimothy R O'Connor
Nov 3, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Olivier ReelfsAntony R Young
Apr 25, 2020·Environmental and Molecular Mutagenesis·Yusuke Kojima, Yuichi J Machida
Nov 4, 2017·Science China. Life Sciences·Naeh L Klages-Mundt, Lei Li
Aug 19, 2016·Molecular and Cellular Biochemistry·Maristela Oliveira PoletiniAna Maria de Lauro Castrucci
Oct 18, 2020·Nature Communications·Alexandra StützerHenning Urlaub
Mar 17, 2007·Cancer Research·Emmanuelle DesprasDenis S F Biard
May 7, 2021·Biochimica Et Biophysica Acta. Proteins and Proteomics·Xiaoying WeiKun Yang

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