PMID: 2509231Oct 1, 1989Paper

Repair of H2O2-induced DNA damage in bovine lens epithelial cell cultures

Experimental Eye Research
A SpectorR R Wang

Abstract

H2O2 concentrations only slightly higher than normal physiological levels found in the lens and aqueous fluid produce a significant number of DNA single-strand breaks in lens epithelial cell cultures. In this investigation, the repair of DNA damaged by short-term, H2O2-induced oxidation was examined in bovine lens epithelial cell cultures. Repair was rapidly initiated and was almost completed in 30 min. A drop in NAD concentration was associated with the DNA damage. 3-Aminobenzamide inhibition of poly(ADP-ribose) polymerase, an enzyme believed to be stimulated by DNA oxidation and involved in DNA repair, prevented the loss of NAD. In contrast, a similar drop in ATP concentration was only slightly lessened by the presence of this inhibitor. Inhibition of the polymerase by 3-aminobenzamide primarily affected only the early recovery period. Overall, recovery occurred almost as effectively in the presence of the inhibitor as in its absence. Preincubation of lens cultures with o-phenanthroline, an iron chelator, prevented the drop in NAD levels associated with DNA damage. Since a hydroxyl radical is produced from H2O2 by a Fenton type reaction, this result supports the concept that the H2O2-induced oxidation of DNA is caused by hydr...Continue Reading

References

Oct 19, 1976·Biochemistry·K W KohnC A Friedman
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A Spector, D Roy
Jul 1, 1979·Photochemistry and Photobiology·M E Hoffmann, R Meneghini
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·I U SchraufstatterC G Cochrane
Aug 15, 1985·The Biochemical Journal·J C Gaal, C K Pearson
Jun 3, 1988·Science·J A Imlay, S Linn
Jan 1, 1988·Journal of Toxicology and Environmental Health·M J Olson
Sep 1, 1987·Experimental Eye Research·A SpectorS Chifflet
Sep 1, 1985·Experimental Eye Research·F J GiblinV N Reddy
Dec 1, 1985·Current Eye Research·A SpectorG M Wang
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R W Hart, R B Setlow
Mar 19, 1970·Biochimica Et Biophysica Acta·A R Lehmann, M G Ormerod
Mar 1, 1969·Biophysical Journal·W Veatch, S Okada
Mar 1, 1980·The Biochemical Journal·M R Purnell, W J Whish
Feb 24, 1984·Biochimica Et Biophysica Acta·A C Mello Filho, R Meneghini
Jan 1, 1983·Analytical Biochemistry·M T KarpT N Lövgren
Dec 1, 1981·Experimental Eye Research·A Spector, W H Garner

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Citations

Jan 1, 1995·Archives of Insect Biochemistry and Physiology·R S Pardini
Jan 1, 1990·Mutation Research·N J KleimanA Spector
Nov 12, 2003·Experimental Eye Research·Wanchao MaAbraham Spector
Dec 12, 2001·European Journal of Biochemistry·K Wang, A Spector
Jan 1, 1996·Photochemistry and Photobiology·D SidjaninG E Woloschak
Aug 26, 1999·The British Journal of Ophthalmology·N OhguroY Tano
Oct 24, 2012·Annals of the ICRP·N J Kleiman
May 29, 1998·Survey of Ophthalmology·C CostagliolaF Fioretti
Mar 6, 2012·International Journal of Biological Macromolecules·Jose A MendozaGustavo Zardeneta
Jul 20, 2007·Experimental Eye Research·Mustafa UnalKazim Devranoğlu
Feb 17, 1999·Experimental Eye Research·Y YangN J Kleiman
Jan 27, 2010·Chemico-biological Interactions·Xin WangChun-Xu Hai
Dec 1, 1990·Current Eye Research·N J KleimanA Spector
Sep 1, 1993·Current Eye Research·D SidjaninJ Reddan
Nov 2, 2007·Radiation Research·Norman J KleimanHoward B Lieberman
May 1, 1993·Current Eye Research·N J Kleiman, A Spector
Aug 2, 2017·Photochemistry and Photobiology·Caroline S CencerFrank J Giblin
Sep 23, 1997·Toxicology and Applied Pharmacology·C M Walsh Clang, M D Aleo

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