Repair of rabbit cartilage defect based on the fusion of rabbit bone marrow stromal cells and Nano-HA/PLLA composite material

Artificial Cells, Nanomedicine, and Biotechnology
Weimin ZhuDaping Wang

Abstract

Objective To assess the effect of the fusion of rabbit bone marrow stromal cells (rBMSCs) and Nano-hydroxyapatite/poly (l-lactic acid) (Nano-HA/PLLA) in repairing the rabbit knee joint with full-thickness cartilage defect. Method The rBMSCs were isolated and cultured in vitro, and the third generation of rBMSCs was co-cultured with the Nano-HA/PLLA to construct the tissue-engineered cartilage (TEC). Eighteen New Zealand white rabbits were selected and randomly divided into three groups, namely, TEC group, Nano-HA/PLLA group, and control group. A cartilage defect model with the diameter of 4.5 mm and depth of 5 mm was constructed on the articular surface of medial malleolus of rabbit femur. General observation, histological observation, and Wakitani's histological scoring were conducted in the 12th and 24th week postoperatively. Results The results of TEC group indicated that new cartilage tissue was formed on the defect site and subchondral bone achieved physiological integration basically. Histological and immunohistochemical analyses indicated the generation of massive extracellular matrix. In contrast, limited regeneration and reconstruction of cartilage was achieved in the Nano-HA/PLLA group and control group, with a signif...Continue Reading

References

Oct 18, 2007·Biopolymers·Julie Glowacki, Shuichi Mizuno
Sep 17, 2010·The Journal of Bone and Joint Surgery. American Volume·Joshua D HarrisDavid C Flanigan
May 21, 2011·Tissue Engineering. Part B, Reviews·A Hari ReddiJosé A Andrades
Nov 24, 2011·Nanoscale Research Letters·Zhao Yan-ZhongZhou Ke-Chao
Aug 7, 2012·Journal of Biomedical Materials Research. Part a·Sargon BarkarmoVictoria Stenport
Aug 21, 2013·Artificial Cells, Nanomedicine, and Biotechnology·Weimin ZhuYanjun Zeng
Dec 26, 2013·In Vitro Cellular & Developmental Biology. Animal·Weimin ZhuYanjun Zeng

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Citations

Sep 26, 2020·Frontiers in Bioengineering and Biotechnology·Joseph G LyonsSilvia Minardi
Mar 27, 2020·Frontiers in Chemistry·Zhongyang LiuPeifu Tang
Apr 7, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Parinaz AbdollahiyanAhad Mokhtarzadeh

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