Repair of Rat Sciatic Nerve Defects by Using Allogeneic Bone Marrow Mononuclear Cells Combined With Chitosan/Silk Fibroin Scaffold

Cell Transplantation
Min YaoJianhui Gu

Abstract

The therapeutic benefits of bone marrow mononuclear cells (BM-MNCs) in many diseases have been well established. To advance BM-MNC-based cell therapy into the clinic for peripheral nerve repair, in this study we developed a new design of tissue-engineered nerve grafts (TENGs), which consist of a chitosan/fibroin-based nerve scaffold and BM-MNCs serving as support cells. These TENGs were used for interpositional nerve grafting to bridge a 10-mm-long sciatic nerve defect in rats. Histological and functional assessments after nerve grafting showed that regenerative outcomes achieved by our developed TENGs were better than those achieved by chitosan/silk fibroin scaffolds and were close to those achieved by autologous nerve grafts. In addition, we used green fluorescent protein-labeled BM-MNCs to track the cell location within the chitosan/fibroin-based nerve scaffold and trace the cell fate at an early stage of sciatic nerve regeneration. The result suggested that BM-MNCs could survive at least 2 weeks after nerve grafting, thus helping to gain a preliminary mechanistic insight into the favorable effects of BM-MNCs on axonal regrowth.

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Citations

Oct 25, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Wei LiuDaping Wang
Oct 30, 2018·Cell Transplantation·Diego Noé Rodríguez SánchezRogério Martins Amorim
Nov 28, 2019·Journal of Biomaterials Science. Polymer Edition·Fangwen ZhaDemei Yu
May 10, 2017·Journal of Tissue Engineering and Regenerative Medicine·Chengbin XueXiaosong Gu
Apr 9, 2020·Frontiers in Bioengineering and Biotechnology·Fengxuan HanBin Li
Feb 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chengbin XueXiaosong Gu
Apr 26, 2019·Frontiers in Cellular Neuroscience·A BoeckerL Harhaus
Jun 5, 2018·Neural Regeneration Research·Nitesh P PatelJason H Huang
Feb 27, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Michael J LandryChristopher J Barrett

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Methods Mentioned

BETA
transgenic
density gradient centrifugation
flow cytometry
light microscopy
fluorescence microscopy

Software Mentioned

CatWalk
CatWalk XT
Image Pro Plus
Leica QWin

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