PMID: 8604332Mar 15, 1996Paper

Repair of rDNA in Saccharomyces cerevisiae: RAD4-independent strand-specific nucleotide excision repair of RNA polymerase I transcribed genes

Nucleic Acids Research
R A VerhageJ Brouwer

Abstract

Removal of UV-induced pyrimidine dimers from the individual strands of the rDNA locus in Saccharomyces cerevisiae was studied. Yeast rDNA, that is transcribed by RNA polymerase I(RNA pol I), is repaired efficiently, slightly strand-specific and independently of RAD26, which has been implicated in transcription-coupled repair of the RNA pol II transcribed RPB2 gene. No repair of rDNA is observed in rad1,2,3 and 14 mutants, demonstrating that dimer removal from this highly repetitive DNA is accomplished by nucleotide excision repair (NER). In rad7 and rad16 mutants, which are specifically deficient in repair of non-transcribed DNA, there is a clear preferential repair of the transcribed strand of rDNA, indicating that strand-specific and therefore probably transcription-coupled repair of RNA pol I transcribed genes does exist in yeast. Unexpectedly, the transcribed but not the non-transcribed strand of rDNA can be repaired in rad4 mutants, which seem otherwise completely NER-deficient.

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Citations

Sep 12, 2003·DNA Repair·Vasilissa I Manova, Lubomir M Stoilov
Jul 16, 1997·The EMBO Journal·A J van GoolJ H Hoeijmakers
Nov 2, 2005·Molecular and Cellular Biology·Antonio ConconiMichael J Smerdon
Feb 24, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Maxime TremblayAntonio Conconi
Mar 18, 2000·Biochemical and Biophysical Research Communications·H BurgerK Nooter
Jan 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Antonio ConconiMichael J Smerdon
Dec 2, 2008·DNA Repair·Raymond WatersSimon H Reed
Aug 26, 2006·Mutation Research·Vasilissa ManovaLubomir Stoilov
Feb 2, 2002·Nature Reviews. Molecular Cell Biology·Jesper Q Svejstrup
Dec 5, 1998·The Journal of Biological Chemistry·L E JansenJ Brouwer
Jul 4, 2018·Proceedings of the National Academy of Sciences of the United States of America·Laurianne DanielGiuseppina Giglia-Mari
Jan 1, 1997·Molecular and Cellular Biology·R Dammann, G P Pfeifer

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