Repair of spinal cord injury by implantation of bFGF-incorporated HEMA-MOETACL hydrogel in rats

Scientific Reports
Bo ChenDingjun Hao

Abstract

There is no effective strategy for the treatment of spinal cord injury (SCI). An appropriate combination of hydrogel materials and neurotrophic factor therapy is currently thought to be a promising approach. In this study, we performed experiments to evaluate the synergic effect of implanting hydroxyl ethyl methacrylate [2-(methacryloyloxy)ethyl] trimethylammonium chloride (HEMA-MOETACL) hydrogel incorporated with basic fibroblast growth factor (bFGF) into the site of surgically induced SCI. Prior to implantation, the combined hydrogel was surrounded by an acellular vascular matrix. Sprague-Dawley rats underwent complete spinal cord transection at the T-9 level, followed by implantation of bFGF/HEMA-MOETACL 5 days after transection surgery. Our results showed that the bFGF/HEMA-MOETACL transplant provided a scaffold for the ingrowth of regenerating tissue eight weeks after implantation. Furthermore, this newly designed implant promoted both nerve tissue regeneration and functional recovery following SCI. These results indicate that HEMA-MOETACL hydrogel is a promising scaffold for intrathecal, localized and sustained delivery of bFGF to the injured spinal cord and provide evidence for the possibility that this approach may have...Continue Reading

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Citations

Jan 16, 2016·Cellular and Molecular Life Sciences : CMLS·John BiancoAnne des Rieux
Sep 15, 2016·Surgical Neurology International·Ali H PalejwalaAndrew Jea
Feb 10, 2017·ACS Applied Materials & Interfaces·Qingqing WangJian Xiao
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Apr 17, 2021·Molecular Neurobiology·Lars Klimaschewski, Peter Claus
Dec 19, 2018·Bioconjugate Chemistry·Mozhdeh ImaninezhadSilviya Petrova Zustiak
Dec 9, 2019·ACS Biomaterials Science & Engineering·Heta ThakarAkshay Srivastava

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Methods Mentioned

BETA
fluorescence microscopy
scanning electron microscopy
transmission electron microscopy
dissecting

Software Mentioned

SPSS16
Neurolucida

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