Repair of the injury to respiratory epithelial cells characteristic of asthma is stimulated by Allomyrina dichotoma agglutinin specific serum glycoproteins

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
B J Patchell, D R Dorscheid

Abstract

The airway epithelium acts as a protective barrier, separating the external environment from the underlying tissue. Daily challenges result in damage to the epithelium that, in normal individuals, is quickly and effectively repaired. In respiratory diseases, such as asthma, this repair is compromised. Following injury to the epithelium, plasma leaks into the airway lumen acting as a protective protein cap. Carbohydrates are mediators of epithelial repair, however, the associated regulatory proteins remain unknown. To identify mediators of epithelial repair based on their carbohydrate moieties using an in vitro wound repair culture model of human airway epithelial cells (1HAEo(-)). Using the lectin Allomyrina dichotoma agglutinin (AlloA) as a tool, ligands essential in the repair of damaged epithelium were characterized. AlloA was subsequently used to purify and identify a glycoprotein associated with epithelial repair. The addition of AlloA to the media of mechanically wounded monolayers inhibited repair. Fetuin, a highly glycosylated serum protein, was identified as a glycoprotein bound by AlloA. The addition of fetuin to serum starved monolayers stimulated wound closure. These results indicate that following mechanical injury...Continue Reading

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Citations

Apr 15, 2010·Journal of Toxicology and Environmental Health. Part a·Nicholas D ManzoJanice A Dye
Jun 19, 2010·American Journal of Respiratory Cell and Molecular Biology·Tanveer AhmadAnurag Agrawal
May 3, 2006·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P M Lackie, E C Adam
Oct 10, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Elizabeth C AdamPeter M Lackie
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Oct 19, 2007·The Journal of Biological Chemistry·Ronald L Mellgren, Xinhua Huang
Aug 4, 2020·Current Pharmaceutical Biotechnology·Rana KameiSenjam S Singh

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