Repair potential of nonsurgically delivered induced pluripotent stem cell-derived chondrocytes in a rat osteochondral defect model

Journal of Tissue Engineering and Regenerative Medicine
Yeri Alice RimJi Hyeon Ju

Abstract

Human induced pluripotent stem cells (hiPSCs) are thought to be an alternative cell source for future regenerative medicine. hiPSCs may allow unlimited production of cell types that have low turnover rates and are difficult to obtain such as autologous chondrocytes. In this study, we generated hiPSC-derived chondrogenic pellets, and chondrocytes were isolated. To confirm the curative effects, chondrogenic pellets and isolated chondrocytes were transplanted into rat joints with osteochondral defects. Isolated hiPSC-derived chondrocytes were delivered in the defect by a single intra-articular injection. The generated hiPSC-derived chondrogenic pellets had increased chondrogenic marker expression and accumulated extracellular matrix proteins. Chondrocytes were successfully isolated from the pellets. Alcian blue staining and collagen type II were detected in the cells. Chondrogenic marker expression was also increased in the isolated cells. Transplanted chondrogenic pellets and chondrocytes both had curative effects in the osteochondral defect rat model. Detection of human proteins in the joints proved that the cells were successfully delivered into the defect. Chondrogenic pellets or chondrocytes generated from hiPSCs have potenti...Continue Reading

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Citations

Nov 8, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Rui DongPinger Wang
Jan 8, 2021·Development, Growth & Differentiation·Akihiro Yamashita, Noriyuki Tsumaki
Feb 13, 2021·International Journal of Molecular Sciences·Shoichiro TaniHironori Hojo
Dec 31, 2020·Life·Yeri Alice Rim, Ji Hyeon Ju
Feb 14, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Matthew WalkerMarco Cantini
Feb 26, 2021·Stem Cells International·Shuangpeng JiangQuanyi Guo
Mar 7, 2021·Biomedicines·Maria CsobonyeiovaLubos Danisovic
Apr 13, 2021·Frontiers in Bioengineering and Biotechnology·Xia ZhaoAravind Athiviraham

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