PMID: 321311Jan 1, 1977Paper

Repair, replication and survival in uv-irradiated Escherichia coli

Folia Microbiologica
M Sedliaková, V Slezáriková

Abstract

The influence of dimer removal through excision or photoreactivation on the kinetics of DNA synthesis, sedimentation profiles of DNA molecules and survival of cells was investigated in excision-deficient and excision-proficient Escherichia coli K-12 after a flux of 20 J M-2. In excision-deficient cells photoreactivation did not influence the kinetics of DNA synthesis for a long period and the sedimentation properties of DNA synthesized immediately after photoreactivation were influenced only slightly. However, survival was increased remarkably. In excision-proficient cells where dimers were removed through excision, the kinetics of DNA synthesis increased rapidly, normal-sized DNA molecules were synthesized 60 min after irradiation and survival was substantially higher than in the above-mentioned case. This can hardly be interpreted as a more complete repair of dimers by excision because the persistence of dimers in these cells did not significantly influence either the kinetics of DNA synthesis or normalization of DNA molecules and/or survival of cells. It is concluded that persisting dimers play an important role in excision-deficient but not in excision-proficient cells, that a non-dimer damage to DNA causes inhibition of DN...Continue Reading

References

Jan 1, 1977·Folia Microbiologica·M SedliakováM Pirsel
Mar 1, 1977·Photochemistry and Photobiology·M SedliakováV Slezáriková
Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S G Sedgwick
Nov 1, 1975·Biophysical Journal·E C Pollard, P M Achey
Aug 21, 1975·Biochimica Et Biophysica Acta·A R Lehmann, S Stevens
Oct 1, 1974·Biophysical Journal·S N BuhlJ D Regan
Nov 8, 1972·Nature: New Biology·B A Bridges
May 31, 1974·Biochimica Et Biophysica Acta·M SedliakováV Slezáriková
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·P Howard-FlandersR S Cole
Apr 14, 1969·Journal of Molecular Biology·H Michalke, H Bremer
Jan 1, 1969·Annual Review of Microbiology·E M Witkin
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May 28, 1972·Journal of Molecular Biology·A R Lehmann
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Apr 1, 1967·Journal of Bacteriology·A B Pardee, L S Prestidge
Jul 23, 1969·Biochemical and Biophysical Research Communications·J E Cleaver, G H Thomas

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