Repeat-induced gene silencing in mammals

Nature Genetics
D GarrickE Whitelaw

Abstract

In both plants and Drosophila melanogaster, expression from a transgenic locus may be silenced when repeated transgene copies are arranged as a concatameric array. This repeat-induced gene silencing is frequently manifested as a decrease in the proportion of cells that express the transgene, resulting in a variegated pattern of expression. There is also some indication that, in transgenic mammals, the number of transgene copies within an array can exert a repressive influence on expression, with several mouse studies reporting a decrease in the level of expression per copy as copy number increases. However, because these studies compare different sites of transgene integration as well as arrays with different numbers of copies, the expression levels observed may be subject to varying position effects as well as the influence of the multicopy array. Here we describe use of the lox/Cre system of site-specific recombination to generate transgenic mouse lines in which different numbers of a transgene are present at the same chromosomal location, thereby eliminating the contribution of position effects and allowing analysis of the effect of copy number alone on transgene silencing. Reduction in copy number results in a marked increa...Continue Reading

References

Aug 1, 1991·Molecular & General Genetics : MGG·O Mittelsten ScheidI Potrykus
Jan 1, 1988·Genes & Development·B P Davis, R J MacDonald
Jan 1, 1986·Annual Review of Genetics·R D Palmiter, R L Brinster
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R H Hoess, K Abremski
Sep 6, 1982·FEBS Letters·S Urieli-ShovalA Razin
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·G RobertsonE Whitelaw
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·K ArakiP Vassalli
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·I IngelbrechtA Depicker
Aug 2, 1994·Molecular & General Genetics : MGG·F NeuhuberM A Matzke
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·E H Bresnick, G Felsenfeld
Jul 1, 1993·The Plant Journal : for Cell and Molecular Biology·P MeyerI Niedenhof
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J A SharpeW G Wood
Sep 1, 1993·Plant Molecular Biology·F F AssaadE R Signer
Apr 15, 1996·Nucleic Acids Research·G RobertsonE Whitelaw
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·M LaksoH Westphal
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·F Ye, E R Signer
Nov 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D I Martin, E Whitelaw
Mar 1, 1997·Molecular and Cellular Biology·H G SutherlandE Whitelaw
Jan 1, 1997·Transgenic Research·D R Dorer

❮ Previous
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Citations

Sep 2, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S Henikoff
Mar 10, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D Schindelhauer
Jun 22, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·V Colot, J L Rossignol
Aug 26, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M A MatzkeA J Matzke
Sep 25, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F Lyko, R Paro
Dec 7, 2000·Genes, Chromosomes & Cancer·A CottageM Stanley
Sep 25, 2001·The Journal of Pathology·A M JubbP Quirke
Oct 13, 2007·Journal of Molecular Evolution·Chauncey W Bowers, Judith Singer-Sam
Aug 10, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Jason D Heaney, Sarah K Bronson
Feb 24, 2010·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Keiichiro SuzukiKohnosuke Mitani
Mar 31, 2010·Transgenic Research·Daniel F CarlsonScott C Fahrenkrug
Aug 31, 2010·Transgenic Research·Jannik E JakobsenJacob Giehm Mikkelsen
Jan 12, 2011·Transgenic Research·Daniel F CarlsonScott C Fahrenkrug
Apr 6, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Ildikó KarcagiIbolya Kiss
Jun 28, 2002·Trends in Neurosciences·Paul F Chapman
Jun 6, 2002·Trends in Genetics : TIG·Niall Dillon, Richard Festenstein
Feb 5, 2000·Biochimica Et Biophysica Acta·S Henikoff
Jun 27, 2001·Mutation Research·M KearnsE Whitelaw
Nov 20, 1998·Current Opinion in Biotechnology·F M Rossi, H M Blau
Oct 6, 1999·Current Opinion in Biotechnology·D Metzger, R Feil
Apr 8, 2000·Current Opinion in Genetics & Development·R Festenstein, D Kioussis
Apr 8, 2000·Current Opinion in Genetics & Development·J A BirchlerU Bhadra
Dec 23, 1999·Current Opinion in Genetics & Development·A F Smit
Aug 11, 1998·Trends in Biochemical Sciences·J Bender
Nov 13, 2008·Cytotechnology·C B WhitelawJ Webster
Jan 20, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Amit C NathwaniAndrew M Davidoff
Jan 13, 1998·Nature Genetics·A P Wolffe
Dec 26, 2001·Nature Genetics·Bong June YoonPaul D Soloway
Dec 3, 2011·Nature Protocols·Maximiliano L SusterKoichi Kawakami

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