Repeated allergen inhalations induce DNA synthesis in airway smooth muscle and epithelial cells in vivo.

The American Journal of Physiology
R A PanettieriJ G Martin

Abstract

Airway smooth muscle (ASM) mass appears to be increased in the bronchi of patients with chronic severe asthma. Although the precise mechanisms that induce these changes are unknown, increases in ASM mass are caused, in part, by ASM cell proliferation. After allergen challenge in rats, it has been possible to demonstrate an increase in ASM mass by morphometric techniques. To examine whether hyperplasia is involved in ASM cell growth in vivo, we investigated whether repeated allergen challenges in sensitized Brown Norway rats stimulated DNA synthesis in airway epithelial and ASM cells. Animals that were actively sensitized to ovalbumin (OA) received either three aerosolized OA or saline challenges at 5-day intervals. DNA synthesis was measured by indirect immunohistochemical techniques with an anti-bromodeoxyuridine (BrdU) antibody. OA inhalations increased ASM mass as determined by morphometry and also induced DNA synthesis in both airway epithelial and ASM cells in the airways of sensitized animals compared with saline-challenged control animals. ASM mass was increased in large- and medium-sized airways but not in small airways. However, the number of BrdU-positive ASM cells normalized to basement membrane length was also great...Continue Reading

References

Aug 1, 1991·The American Review of Respiratory Disease·S SapienzaJ G Martin
Aug 1, 1990·American Journal of Respiratory Cell and Molecular Biology·A ClementJ S Brody
Jan 1, 1989·The American Review of Respiratory Disease·A L JamesJ C Hogg
Oct 1, 1988·Journal of Applied Physiology·S Bellofiore, J G Martin
Sep 1, 1987·The Journal of Allergy and Clinical Immunology·J Kleinerman, L Adelson
Feb 1, 1984·Thorax·P J BrownK E Finucane
Sep 1, 1994·American Journal of Respiratory and Critical Care Medicine·J M PilewskiS M Albelda
Sep 1, 1994·American Journal of Respiratory Cell and Molecular Biology·M B HershensonJ Solway
Aug 1, 1993·The American Review of Respiratory Disease·C G WangJ G Martin
Jan 1, 1993·The American Review of Respiratory Disease·J G MartinW S Powell

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Citations

Jul 3, 2003·Médecine sciences : M/S·Laurent Benayoun, Marina Pretolani
Aug 4, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Isabelle LabontéJames G Martin
Apr 23, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·S SiddiquiJ G Martin
Apr 6, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shawnessy D LarsonEdward S Schelegle
Nov 15, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·A TrifilieffC Bertrand
Oct 22, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Lyn M MoirStuart J Hirst
Nov 18, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·J Kelley BentleyMarc B Hershenson
Apr 3, 2007·American Journal of Respiratory and Critical Care Medicine·Pierre CamaterosDanuta Radzioch
Sep 28, 2004·American Journal of Respiratory and Critical Care Medicine·Kristina Rydell TörmänenJonas S Erjefält
Feb 3, 2007·American Journal of Respiratory Cell and Molecular Biology·Clare G McVickerStuart J Hirst
Oct 1, 2003·Respiratory Physiology & Neurobiology·Limei Zhou, Marc B Hershenson
Dec 13, 2005·Pulmonary Pharmacology & Therapeutics·James G Martin, Meiyo Tamaoka

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