PMID: 8602177Mar 26, 1996Paper

Repeated analysis of sister chromatid exchange induction by diepoxybutane in cultured human lymphocytes: effect of glutathione S-transferase T1 and M1 genotype

Mutation Research
S LandiR Barale

Abstract

Spontaneous and diepoxybutane (DEB)-induced sister-chromatid exchanges (SCEs) were examined in whole-blood lymphocyte cultures of 3 men and 4 women. A strong increase in mean number of SCEs per cell with increasing DEB concentrations (0, 2, and 4 microM) was observed in cultures of all subjects, but 3 of the donors were clearly more sensitive than the others. The SCE measurements were repeated 2-6 times per donor over a period of 55 months to assess the stability of the individual SCE response. The results showed that SCE induction by DEB was steady in the individuals during the follow-up at each DEB dose, with no significant differences among the repeated experiments. At 4 microM DEB, the DEB-sensitive and -resistant donors could be reliably be differentiated from each other in all trials. As DEB-sensitivity has been suggested to be due to the lack of glutathione S-transferase (GST) T1, the donors were genotyped for the presence of GSTT1 and GSTM1 genes. The 3 individuals found to be DEB-sensitive were all of the GSTT1 null genotype, whereas the 4 DEB-resistant donors were GSTT1 positive, which supported the role of the GSTT1 gene in determining DEB-sensitivity. Three of the DEB-resistant and none of the DEB-sensitive had the ...Continue Reading

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Citations

Jul 23, 2008·Bulletin of Experimental Biology and Medicine·S S AlexaninM N Timofeeva
Dec 4, 2003·Mutation Research·Hannu Norppa
Apr 20, 2004·Toxicology Letters·Hannu Norppa
Feb 13, 2007·Environmental Health : a Global Access Science Source·Eckart SchnakenbergWerner Schloot
Sep 22, 2005·British Journal of Haematology·Stella M DaviesArleen D Auerbach
Nov 19, 2015·European Journal of Nutrition·Ping LiuC D'Arcy J Holman
Mar 4, 2015·Pediatric Blood & Cancer·Giovanni PaganoFederico V Pallardó
Apr 25, 2017·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Alfredo SantovitoPiero Cervella
Sep 28, 2010·Critical Reviews in Toxicology·Richard J AlbertiniMichael L Gargas
Mar 7, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S M DaviesJ P Perentesis
Dec 7, 2011·Human & Experimental Toxicology·O DalmizrakN Ozer

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