Repeated Duplication of Argonaute2 Is Associated with Strong Selection and Testis Specialization in Drosophila

Genetics
Samuel H LewisDarren J Obbard

Abstract

Argonaute2 (Ago2) is a rapidly evolving nuclease in the Drosophila melanogaster RNA interference (RNAi) pathway that targets viruses and transposable elements in somatic tissues. Here we reconstruct the history of Ago2 duplications across the D. obscura group and use patterns of gene expression to infer new functional specialization. We show that some duplications are old, shared by the entire species group, and that losses may be common, including previously undetected losses in the lineage leading to D. pseudoobscura We find that while the original (syntenic) gene copy has generally retained the ancestral ubiquitous expression pattern, most of the novel Ago2 paralogs have independently specialized to testis-specific expression. Using population genetic analyses, we show that most testis-specific paralogs have significantly lower genetic diversity than the genome-wide average. This suggests recent positive selection in three different species, and model-based analyses provide strong evidence of recent hard selective sweeps in or near four of the six D. pseudoobscura Ago2 paralogs. We speculate that the repeated evolution of testis specificity in obscura group Ago2 genes, combined with their dynamic turnover and strong signatur...Continue Reading

References

Mar 10, 2018·Viruses·William H PalmerRonald P van Rij
Jun 21, 2018·Molecular Biology and Evolution·Quentin Helleu, Mia T Levine
Jul 23, 2019·PLoS Genetics·Christopher Ellison, Doris Bachtrog
Oct 24, 2019·Proceedings. Biological Sciences·Cécile CourretAmanda M Larracuente
Oct 20, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Rudi L VerspoorNina Wedell

Related Concepts

Meiotic Cell Cycle
Study
Biochemical Pathway
Benign Neoplasm of Testis
AGO2 gene
PIWI Subfamily of Argonaute Proteins
Virus
Genome
EIF2C2 protein, human
Neoplasm of Uncertain or Unknown Behavior of Testis

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