Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice using the flocculating yeast Saccharomyces cerevisiae F118 strain

Bioresource Technology
Hans WijayaAkihiko Kondo

Abstract

The aim of this study was to construct a cost-effective method for repeated bioethanol production using membrane (ultrafiltration permeation and nanofiltration concentration)-concentrated sweet sorghum juice by using flocculent Saccharomyces cerevisiae F118 strain. With low initial dry cell concentrations at around 0.28-0.35 g L-1, the S. cerevisiae F118 strain provided an ethanol titer of 86.19 ± 1.15 g L-1 (theoretical ethanol yield of 70.77%), which was higher than the non-flocculent S. cerevisiae BY4741 strain at 33.92 ± 0.99 g L-1 after 24 h fermentation. This result was correlated with higher gene expressions of the sucrose-hydrolysing enzyme invertase, sugar phosphorylation, and pyruvate-to-ethanol pathways in the F118 strain compared with the BY4741 strain. Sequential fed-batch fermentation was conducted, and the F118 strain was easily separated from the fermentation broth via the formation of flocs and sediment. After the 5th cycle of fermentation with the F118 strain, the ethanol concentration reached 100.37 g L-1.

References

Feb 6, 1998·FEMS Microbiology Reviews·E Nevoigt, U Stahl
Sep 26, 2006·Applied and Environmental Microbiology·Hong WuHitoshi Shimoi
Feb 29, 2008·Microbial Cell Factories·Fernanda BadottiBoris U Stambuk
May 16, 2008·FEMS Yeast Research·Olga de SmidtJacobus Albertyn
Jul 7, 2009·Biotechnology Advances·X Q Zhao, F W Bai
Dec 1, 2010·Journal of Applied Microbiology·E V Soares
Feb 7, 2012·Biotechnology Advances·Yi HeBao-Qiang Liao
Apr 10, 2014·TheScientificWorldJournal·Hossain ZabedAmru Nasrulhaq Boyce
Apr 11, 2015·Bioresource Technology·Kengo SasakiAkihiko Kondo

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