Repeated exposure to acid and bile selectively induces colonic phenotype expression in a heterogeneous Barrett's epithelial cell line

Laboratory Investigation; a Journal of Technical Methods and Pathology
Manisha BajpaiKiron M Das

Abstract

Barrett's epithelium is a precancerous, specialized columnar metaplasia in the distal esophagus. We demonstrate the changes in cellular phenotype in a non-neoplastic Barrett's cell line (BAR-T), following exposure to acid and bile salt, the two important components of gastroesophageal refluxate. Cell phenotypes in BAR-T cell line were quantified by fluorescence-activated cell sorting (FACS) using monoclonal antibodies against markers: cytokeratin 8/18 (CK8/18) for columnar, CK4 for squamous, mAbDas-1 for colonic epithelial cell phenotype and p75NTR for esophageal progenitors. Cells were exposed for 5 min each day to 200 microM glycochenodeoxycholic acid at pH 4, pH 6 and pH 7.4 or only to acid (pH 4) for up to 6 weeks. The BAR-T cell line comprised 35+/-5.2% CK8/18, 32+/-3.5% mAbDas-1, 9.5+/-3% CK4 and 4+/-2.5% p75NTR-positive cells. Single exposure to acid and or bile did not change cell phenotypes. However, chronic treatment for at least 2 weeks significantly enhanced (P<0.05) the expression of colonic phenotype and CK8/18-positive cells, as evidenced by FACS analysis. Bile salt at pH 4 and bile salt followed by acid (pH 4) in succession were the strongest stimulators (P<0.01) for induction of colonic phenotype cells. Squamou...Continue Reading

References

Feb 1, 1988·The British Journal of Surgery·P GillenT P Hennessy
Jun 1, 1988·The British Journal of Surgery·P GillenT P Hennessy
Oct 3, 1985·The New England Journal of Medicine·A J CameronW S Payne
Jul 15, 1994·Cancer·G N Stemmermann
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·W KutcheraS M Prescott
Nov 1, 1996·The Journal of Clinical Investigation·R C FitzgeraldG Triadafilopoulos
Apr 16, 1998·QJM : Monthly Journal of the Association of Physicians·P BlokG N Tytgat
Mar 18, 1999·The New England Journal of Medicine·J LagergrenO Nyrén
Nov 11, 1999·Clinical and Experimental Immunology·K V KesariK M Das
Mar 1, 2000·Digestive Diseases and Sciences·L H GriffelK M Das
May 18, 2001·Digestive Diseases·J E Richter
Feb 8, 2002·Gastroenterology·Rhonda F SouzaStuart Jon Spechler
Feb 13, 2002·The American Journal of Pathology·Peter FickertHelmut Denk
Mar 21, 2002·Chest Surgery Clinics of North America·Alan G Casson
Jun 13, 2002·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·J KleeffM W Büchler
Oct 19, 2002·The American Journal of Gastroenterology·Steven R DeMeesterTom R DeMeester
Dec 21, 2002·The American Journal of Gastroenterology·Claudia Rogge-WolfLouis H Griffel
Aug 2, 2003·Scandinavian Journal of Gastroenterology·H KumagaiT Hattori
Sep 23, 2003·The American Journal of Gastroenterology·Robert S FisherKiron M Das
Oct 14, 2003·Current Pharmaceutical Design·E PiazueloA Lanas
Nov 19, 2003·Pathology, Research and Practice·Václav MandysRoberto Revoltella
Apr 20, 2004·The Medical Journal of Australia·Lybus C HillmanAnthony C Clarke
Apr 20, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yinghao SuRaj K Goyal
Jun 25, 2004·Mutagenesis·A J JollyL J Hardie
Jul 3, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Rhonda F SouzaStuart J Spechler

❮ Previous
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Citations

Apr 6, 2012·Cellular Oncology (Dordrecht)·P BusJ W P M van Baal
Dec 4, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Aaron GoldmanKaterina Dvorak
Aug 2, 2014·The Journal of Clinical Investigation·David H WangRhonda F Souza
Oct 15, 2013·Annals of the New York Academy of Sciences·Michael K GibsonManisha Bajpai
Mar 25, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Carlos D MinacapelliKiron M Das
Jul 6, 2018·Annals of the New York Academy of Sciences·Matthew D ReadWayne A Phillips
Sep 2, 2011·Journal of Cellular Physiology·Rachelle E KosoffCarlo C Maley
Jul 23, 2009·Molecular Carcinogenesis·Yan LiRobert C G Martin
Sep 24, 2009·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Dhiren Nehra
Mar 24, 2010·International Journal of Cancer. Journal International Du Cancer·Kiron M DasKim Hirshfield
Jun 6, 2009·International Journal of Food Sciences and Nutrition·Vassiliki Costarelli
Dec 21, 2010·Journal of Gastroenterology and Hepatology·Wayne A PhillipsDavid C Whiteman
Sep 29, 2011·Annals of the New York Academy of Sciences·Hugh BarrPaolo Bechi
Sep 29, 2011·Annals of the New York Academy of Sciences·Dianchun FangAndrew M Bellizzi
Oct 1, 2014·Annals of the New York Academy of Sciences·Manisha BajpaiHenry To
Sep 29, 2011·Annals of the New York Academy of Sciences·Rhonda F SouzaJohn P Lynch
Jul 1, 2018·Cancer Research·Aleksandra M UrbanskaMasoud Mozafari
Jun 9, 2021·Nature Communications·Vincent T JanmaatMaikel P Peppelenbosch
Jun 29, 2021·Frontiers in Genetics·Manisha BajpaiHana Aviv

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