Repeated immobilization stress decreases mRNA and protein levels of the type 1 IP3 receptor in rat heart

Annals of the New York Academy of Sciences
Katarina KrepsovaO Krizanova

Abstract

Stress is one of the major contributors to the development of cardiovascular disorders and psychiatric illnesses. Immobilization stress belongs to severe stressors and is known to activate several calcium transport systems. The aim of this work was to determine whether repeated immobilization stress changes mRNA and protein levels of the type 1 and 2 inositol-1,4,5-trisphosphate (IP(3)) receptors in cardiac tissue. Rats were immobilized for 7 days, 2 h daily. After repeated immobilization, increased numbers of collagen fibers were accumulated in the heart atria compared to hearts of the control group of rats. Gene expression was determined after reverse transcription and subsequent real-time polymerase chain reaction, using SYBR Green fluorescent dye. Protein levels were determined by Western blot and hybridization with the primary antibody against IP(3) receptors. Contrary to single immobilization, repeated immobilization decreased a gene expression of the type 1 and 2 IP(3) receptors, and also protein levels of the IP(3) receptors. Although the physiologic relevance of our observations remains to be elucidated, we propose that the decrease in IP(3) receptors may have an impact on the development of the pathophysiologic change...Continue Reading

References

Jun 1, 1996·Neurodegeneration : a Journal for Neurodegenerative Disorders, Neuroprotection, and Neuroregeneration·L S HaugS I Walaas
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·A A GenazzaniD Guerini
Apr 25, 2000·Cardiovascular Research·T K BorgA M Samarel

❮ Previous
Next ❯

Citations

Sep 22, 2009·Canadian Journal of Physiology and Pharmacology·Adriana AdameovaNaranjan S Dhalla
Nov 7, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·L GavrilovicS Dronjak
Dec 27, 2005·Biochimica Et Biophysica Acta·Dana JurkovicovaOlga Krizanova
Jul 9, 2004·Annals of the New York Academy of Sciences·Olga KrizanovaRichard Kvetnansky
Jan 6, 2009·Annals of the New York Academy of Sciences·Dana JurkovicovaOlga Krizanova
May 22, 2007·Stress : the International Journal on the Biology of Stress·O KrizanovaL Kubovcakova

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.