Repeated Inoculation of Young Calves With Rumen Microbiota Does Not Significantly Modulate the Rumen Prokaryotic Microbiota Consistently but Decreases Diarrhea

Frontiers in Microbiology
Dengpan BuZhongtang Yu

Abstract

The complex rumen microbiota exhibits some degree of host specificity. The undeveloped simple rumen microbiota is hypothetically more amendable. The objective of this study was to investigate if the rumen prokaryotic microbial assemblage of young calves can be reprogrammed by oral inoculation with rumen microbiota of adult cows. Twenty newborn male calves were randomly assigned to four groups (n = 5 per group), with two groups being orally inoculated with rumen microbiota (fresh rumen fluid) collected from two lactating dairy cows, while the other two groups receiving autoclaved rumen fluid collected from another two donor cows. Each calf was orally drenched with 100, 200, 300, 400, and 500 mL of the rumen fluid at d3, d7, d21, d42, and d50, respectively, after birth. The inoculation with rumen microbiota did not affect (P > 0.05) feed intake, average daily gain (ADG), heart girth, or feed conversion ratio but significantly (P < 0.01) lowered instance of diarrhea. At the age of 77 days (27 days post-weaning), all the calves were slaughtered for the sampling of rumen content and determination of empty rumen weight. Rumen fermentation characteristics were not affected (P > 0.05) by the inoculation. Rumen prokaryotic microbiota an...Continue Reading

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Citations

Feb 23, 2021·Computational and Structural Biotechnology Journal·Nida Amin, Jana Seifert
Jun 17, 2021·Animal Health Research Reviews·Gercino Ferreira Virgínio Júnior, Carla Maris Machado Bittar

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Datasets Mentioned

BETA
PRJNA562303

Methods Mentioned

BETA
Assay
PCA

Software Mentioned

R package ggfortify
PERMANOVA
SAS
LEfSe
PICRUSt2
PAST3
Galaxy
DADA2
Cutadapt
R package Venn Diagram

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