PMID: 8594033Nov 1, 1995Paper

Repeated measurement of respiratory function and bronchoconstriction in unanesthetized mice

Journal of Applied Physiology
E M HesselF P Nijkamp

Abstract

A noninvasive forced oscillation technique was used to determine respiratory function in unanesthetized and spontaneously breathing mice. Pseudorandom noise pressure variations in a frequency range of 16-208 Hz were applied to the body surface, and the flow response was measured at the nose. From the pressure-flow relationship, respiratory transfer impedance was calculated. Study of intra-animal variability on a short- and a long-term basis revealed that the real part of respiratory transfer impedance was reproducible within 9%. The imaginary part appeared less reproducible (within 22%). Furthermore, bronchoconstrictive responses were investigated and analyzed by evaluation of respiratory resistance as measured at 16 Hz (Rrs16). During the first 15 min after ovalbumin challenge in ovalbumin-sensitized mice, Rrs16 was significantly increased [49 +/- 7% (SE)]. Inhalation of methacholine in untreated mice induced an increase in Rrs16 of 75 +/- 16% (SE). In saline-challenged animals, no significant changes were observed. This method enables evaluation of long-term respiratory function in mice and appeared to be a sensitive measure for bronchoconstriction.

Citations

Feb 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·T GlaabH Renz
Mar 11, 2003·Journal of Applied Physiology·Jason H T Bates, Charles G Irvin
Sep 18, 2007·Respiratory Research·Thomas GlaabWayne Mitzner
Jun 30, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jason H T BatesCharles G Irvin
May 18, 2001·Journal of Applied Physiology·F PetákC Barazzone-Argiroffo
Jun 22, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G R Zosky, P D Sly
Sep 18, 2002·Journal of Applied Physiology·Zoltán Hantos, Vito Brusasco
Sep 18, 2002·Journal of Applied Physiology·Thomas GlaabHelmut Fabel
Jan 20, 2005·American Journal of Respiratory and Critical Care Medicine·Debora G XistoPatricia R M Rocco
Apr 29, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Herbert Luke OgdenTeal S Hallstrand
Jun 5, 2003·Respiratory Research·Charles G Irvin, Jason H T Bates
Dec 19, 2008·Disease Models & Mechanisms·Anthony T Nials, Sorif Uddin
Nov 26, 2003·Respiratory Physiology & Neurobiology·Rachel A CollinsRakesh K Kumar

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