PMID: 9548073Apr 21, 1998Paper

Repertoire breadth of human CD4+ T cells specific for HIV gp120 and p66 (primary antigens) or for PPD and tetanus toxoid (secondary antigens)

Human Immunology
G Li PiraF Manca

Abstract

Antigen derived peptides bound on MHC class II molecules on presenting cells stimulate specific CD4 lymphocytes that are in a naive state if antigen is given for the first time, or in a memory state if antigen has been previously encountered. In order to compare clonal heterogeneity of the human CD4+ T helper repertoire in primary vs. recall responses, we have generated T cell lines in vitro by repeated stimulation of peripheral lymphocytes with primary or with recall antigens. Clonal heterogeneity was broad in the case of recall response to tetanus toxoid or PPD, with a high frequency of specific precursors (> 100 cells/10(6) lymphocytes). In contrast, T cell lines responsive to primary antigens (HIV gp120 or HIV p66) were oligoclonal as defined by TCR V beta gene usage and by spectratyping, and the precursor frequency was low (< 2 cells/10(6) lymphocytes). Primary T cell lines generated from blood samples drawn at different times from the same donor showed that clones with identical TCR CDR3 region coding sequences were expanded, suggesting that in these individuals a large progeny derived from one single precursor is present, even though a previous encounter with the antigen was not documented. Assuming an even in vivo distr...Continue Reading

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Citations

Oct 12, 2001·Immunology Letters·G Li PiraF Manca
Nov 21, 2009·Journal of Immunological Methods·Janice M MoserDonald R Drake
Jan 31, 2014·Clinical and Vaccine Immunology : CVI·Giuseppina Li PiraFabrizio Manca
Mar 16, 2002·Gene Therapy·G PalùF Manca
Jul 20, 2007·Clinical and Vaccine Immunology : CVI·Catherine BarbeyFrançois Spertini
Mar 9, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·D FenoglioF Manca
Sep 26, 2002·Clinical and Experimental Immunology·M D Bodman-SmithA Zumla
Feb 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jason K WhitmireJ Lindsay Whitton

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