Repertoire of Escherichia coli agonists sensed by innate immunity receptors of the bovine udder and mammary epithelial cells.

Veterinary Research
Adeline PorcheriePierre Germon

Abstract

Escherichia coli is a frequent cause of clinical mastitis in dairy cows. It has been shown that a prompt response of the mammary gland after E. coli entry into the lumen of the gland is required to control the infection, which means that the early detection of bacteria is of prime importance. Yet, apart from lipopolysaccharide (LPS), little is known of the bacterial components which are detected by the mammary innate immune system. We investigated the repertoire of potential bacterial agonists sensed by the udder and bovine mammary epithelial cells (bMEC) during E. coli mastitis by using purified or synthetic molecular surrogates of bacterial agonists of identified pattern-recognition receptors (PRRs). The production of CXCL8 and the influx of leucocytes in milk were the readouts of reactivity of stimulated cultured bMEC and challenged udders, respectively. Quantitative PCR revealed that bMEC in culture expressed the nucleotide oligomerization domain receptors NOD1 and NOD2, along with the Toll-like receptors TLR1, TLR2, TLR4, and TLR6, but hardly TLR5. In line with expression data, bMEC proved to react to the cognate agonists C12-iE-DAP (NOD1), Pam3CSK4 (TLR1/2), Pam2CSK4 (TLR2/6), pure LPS (TLR4), but not to flagellin (TLR5)....Continue Reading

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Citations

Jul 25, 2013·Cell and Tissue Research·Ken KobayashiHaruto Kumura
Jan 20, 2012·Journal of Proteome Research·Stine L BislevEmøke Bendixen
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Feb 13, 2013·Veterinary Medicine International·Lorraine M Sordillo
Nov 12, 2013·BMC Veterinary Research·Silja Avall-JääskeläinenSuvi Taponen
Feb 9, 2011·Acta Veterinaria Scandinavica·Piret KalmusKalle Kask
Jul 30, 2015·BMC Veterinary Research·Isabel BauerHans-Martin Seyfert
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Feb 6, 2018·Journal of Dairy Science·Lorraine M Sordillo
Dec 20, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Adeline PorcheriePascal Rainard
Jan 26, 2016·PloS One·Florent KempfPierre Germon
Apr 23, 2016·PloS One·Pascal RainardFlorence B Gilbert

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Methods Mentioned

BETA
PCR
ELISA

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