PMID: 377009Jan 10, 1979Paper

Repetition of tetracycline resistance determinant genes on R plasmid pRSD1 in Escherichia coli

Molecular & General Genetics : MGG
R MattesR Schmitt

Abstract

The 30 megadalton (Mdal)-conjugative, fi- plasmid pRSD1 determines inducible tetracycline resistance (Tc) in Escherichia coli. As shown by restriction analysis, a 3.5 Mdal-EcoRI fragment of pRSD1 spliced into the small plasmid pRSD2124 comprises the entire Tc determinant (tet) region. A restriction map of pRSD1 is presented which includes the location of the tet region and of an "underwound" loop not related to Tc (Burkardt et al., 1978). Selective amplification of tet genes is demonstrated by three lines of evidence. (i) The resistance level of cell harbouring pRSD1 increases approximately tenfold by induction with 10 microgram/ml of tetracycline. Further growth in the presence of 100 microgram/ml of the drug ("tetracycline stress") selects for cells with even higher resistance levels (about 300 microgram/ml) in rec+ cells. In a recA strain, a smaller proportion of cells attains these high resistance levels suggesting the involvement of host recombination. (ii) Electron micrographs of pRSD1-DNA isolated from tetracycline-stressed cells reveal a heterogeneous population of circular DNA molecules ranging between 1.7 and 21.6 micron. The distribution of contour lengths shows a discrete pattern ascribed to the presence of autonomo...Continue Reading

References

Dec 1, 1977·Journal of Bacteriology·D P TaylorR H Rownd
Nov 1, 1977·Plasmid·J GrinstedM H Richmond
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D B ClewellB Bauer
Dec 30, 1975·Molecular & General Genetics : MGG·M SoS Falkow
Apr 2, 1975·Biochimica Et Biophysica Acta·G O HumphreysE S Anderson
May 19, 1972·Science·J E Davies, R Rownd

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Citations

Nov 1, 1990·Molecular Biology Reports·J F Sarkari, S K Mahajan
Apr 18, 2002·FEMS Microbiology Letters·Lucia P FerreiraManoel Victor F Lemos
Jul 18, 2009·Nature Reviews. Microbiology·Linus Sandegren, Dan I Andersson
Sep 1, 1988·Antimicrobial Agents and Chemotherapy·M E TolmaskyP M Marini
Mar 25, 1983·Journal of Molecular Biology·M Chandler, D J Galas
Dec 1, 1985·Journal of Bacteriology·B C Peterson, R H Rownd
Mar 1, 1986·Journal of Bacteriology·I Goldberg, J J Mekalanos
Jul 1, 1980·Cell·M P Calos, J H Miller

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