Repetitive prenatal glucocorticoid therapy reduces oxidative stress in the lungs of preterm lambs

Journal of Applied Physiology
F J WaltherM Ikegami

Abstract

Repetitive courses of maternal prenatal glucocorticoids are often used in high-risk pregnancies with threatening preterm labor to induce lung maturation, but the effects on the cellular oxidant-antioxidant balance in the fetal lung have not been evaluated. We investigated the effect of repetitive treatment with glucocorticoids, beginning early in gestation, on oxidative stress in the preterm ovine lung. Pregnant ewes were randomized to receive one, two, three, or four doses of 0.5 mg/kg betamethasone or saline placebo at 7-day intervals on 104, 111, 118, and 124 days gestation (n = 11 for each group). All lambs were delivered preterm at 125 days gestation, and lung tissue was assayed for antioxidant enzymes, lipid hydroperoxides, and carbonyl proteins. Lung manganese superoxide dismutase, catalase, and glutathione peroxidase activity increased after 1 dose of betamethasone given at 104 days gestation, whereas copper-zinc superoxide dismutase activity increased after 2 doses given at 104 and 111 days gestation. The activity of all four antioxidant enzymes further increased with additional doses and was maximal after four doses of betamethasone. Lung lipid hydroperoxide levels and carbonyl protein content decreased stepwise after...Continue Reading

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Citations

Sep 16, 2000·American Journal of Respiratory and Critical Care Medicine·J E BuntL J Zimmermann
May 29, 2002·Pediatric Research·Machiko Ikegami, Alan H Jobe
Apr 11, 2003·Clinical Obstetrics and Gynecology·Kellie Murphy, Fariba Aghajafari
Dec 4, 2014·Journal of Developmental Origins of Health and Disease·C J D McKinlayJ E Harding
Jun 30, 2005·Biology of the Neonate·L J I ZimmermannV P Carnielli
May 7, 2009·Journal of Perinatology : Official Journal of the California Perinatal Association·V P CarnielliP E Cogo
Apr 15, 2016·Molecular and Cellular Pediatrics·Matthias C HüttenBoris W Kramer
Jun 28, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Junjing JiaNancy E Lane
Apr 11, 2003·Clinical Obstetrics and Gynecology·Alan H Jobe
Oct 21, 2006·Respirology : Official Journal of the Asian Pacific Society of Respirology·Anna M SadowskaWilfried A De Backer
Aug 4, 2009·Antioxidants & Redox Signaling·Máximo VentoAna Gimeno
Jan 15, 2014·The Proceedings of the Nutrition Society·Jane L Tarry-Adkins, Susan E Ozanne
Feb 13, 2001·Clinical Transplantation·J L CracowskiG Bessard
Mar 21, 2007·Obstetrical & Gynecological Survey·Clarissa BonannoRonald J Wapner

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