Repetitive restriction of muscle blood flow enhances mTOR signaling pathways in a rat model

Heart and Vessels
Toshiaki NakajimaYutaka Kano

Abstract

Skeletal muscle is a plastic organ that adapts its mass to various stresses by affecting pathways that regulate protein synthesis and degradation. This study investigated the effects of repetitive restriction of muscle blood flow (RRMBF) on microvascular oxygen pressure (PmvO2), mammalian target of rapamycin (mTOR) signaling pathways, and transcripts associated with proteolysis in rat skeletal muscle. Eleven-week-old male Wistar rats under anesthesia underwent six RRMBF consisting of an external compressive force of 100 mmHg for 5 min applied to the proximal portion of the right thigh, each followed by 3 min rest. During RRMBF, PmvO2 was measured by phosphorescence quenching techniques. The total RNA and protein of the tibialis anterior muscle were obtained from control rats, and rats treated with RRMBF 0-6 h after the stimuli. The protein expression and phosphorylation of various signaling proteins were determined by western blotting. The mRNA expression level was measured by real-time RT-PCR analysis. The total muscle weight increased in rats 0 h after RRMBF, but not in rats 1-6 h. During RRMBF, PmvO2 significantly decreased (36.1 ± 5.7 to 5.9 ± 1.7 torr), and recovered at rest period. RRMBF significantly increased phosphoryl...Continue Reading

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Citations

Oct 21, 2016·Muscle & Nerve·Mizuki SudoYutaka Kano
Mar 3, 2017·Clinical Physiology and Functional Imaging·Matthew B JesseeJeremy P Loenneke
Jan 30, 2019·Medicine and Science in Sports and Exercise·Jean NyakayiruLex B Verdijk
Nov 7, 2018·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Toshiharu NatsumeHisashi Naito
Feb 12, 2020·The American Journal of Sports Medicine·Austin J RammeAsheesh Bedi
Oct 3, 2017·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Kazuho Sakamoto
Nov 3, 2020·The American Journal of Sports Medicine·Austin J RammeAsheesh Bedi

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