PMID: 9431949Feb 12, 1998Paper

Repetitive sequence-based PCR versus pulsed-field gel electrophoresis for typing of Enterococcus faecalis at the subspecies level

Journal of Clinical Microbiology
K MalathumB E Murray

Abstract

Repetitive sequence-based PCR was compared to pulsed-field gel electrophoresis (PFGE) for the ability to discriminate Enterococcus faecalis isolates at the subspecies level. The BOXA2R primer, derived from repetitive sequences in Streptococcus pneumoniae, was applied to 41 isolates of E. faecalis collected from various sources. The REP1R-Dt and REP2-Dt primers, derived from the gram-negative repetitive extragenic palindromic element, were also applied to 18 selected isolates. Of the 41 isolates examined, 7 were beta-lactamase producing and 8 were vancomycin resistant. By PFGE, 17 isolates had distinct patterns; the other 24 were classified into eight different clonal groups. By PCR using the BOXA2R primer, 16 isolates generated distinct patterns; the other 25 were classified into nine different clonal groups. There were only minor differences in the PCR results obtained by using the BOXA2R primer and the REP1R-Dt and REP2-Dt primers. Two isolates among vancomycin-resistant enterococci from the greater Houston, Tex., area were related by PFGE, distinct by PCR with the BOXA2R primer, and related by PCR with the REP1R-Dt and REP2-Dt primers. Clonal relationships among the remaining 39 isolates were similar by both PFGE and PCR. PC...Continue Reading

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Citations

Dec 24, 2004·Antimicrobial Agents and Chemotherapy·Kavindra V Singh, Barbara E Murray
May 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·C D SinigallianoH M Solo-Gabriele
Oct 26, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Monika Eliza ŁysakowskaMonika Sienkiewicz
Mar 5, 2013·Applied and Environmental Microbiology·Qinghong RanMichael J Sadowsky
Jul 3, 2008·Journal of Food Protection·Spiros ParamithiotisEleftherios H Drosinos
Jun 2, 2009·Canadian Journal of Microbiology·Isabelle Proudy
Dec 3, 2013·Indian Journal of Microbiology·Monika Eliza LysakowskaMonika Sienkiewicz
Jun 18, 2020·Antimicrobial Resistance and Infection Control·Ronella MaromVered Schechner
Oct 26, 2000·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J Bedendo, A C Pignatari
Feb 28, 2007·The Journal of General and Applied Microbiology·Dusan JurkovicMarc Vancanneyt
Apr 6, 2013·Journal of Applied Microbiology·M N A AmalM Nur-Nazifah
Oct 1, 2011·Environmental Microbiology Reports·Christopher StaleyValerie J Harwood
Apr 9, 2001·Critical Care Medicine·D K Warren, V J Fraser
Apr 30, 2009·Annual Review of Phytopathology·Isaac Barash, Shulamit Manulis-Sasson
Oct 19, 2017·Journal of Water and Health·Maria Laura GamberoJesica Giuliano Albo
Jun 13, 2020·BMC Microbiology·Dragica DamnjanovicWallace J Bridge
Oct 15, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Esteban C NanniniBarbara E Murray
Jun 2, 2005·FEMS Microbiology Letters·Pavel SvecJean Swings
Apr 2, 1999·Antimicrobial Agents and Chemotherapy·K MalathumB E Murray
Oct 7, 2005·Journal of Clinical Microbiology·Preeti PancholiPhyllis Della Latta
Dec 4, 2012·Microbiology and Molecular Biology Reviews : MMBR·Muruleedhara N ByappanahalliValerie J Harwood
Nov 4, 2000·Journal of Clinical Microbiology·D TurabelidzeA Sulakvelidze

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