PMID: 2119802Jul 10, 1990Paper

Replacement of a cis proline simplifies the mechanism of ribonuclease T1 folding

Biochemistry
T KiefhaberF X Schmid

Abstract

The refolding of ribonuclease T1 is dominated by two major slow kinetic phases that show properties of proline isomerization reactions. We report here that the molecular origin of one of these processes is the trans----cis isomerization of the Ser54-Pro55 peptide bond, which is cis in the native protein but predominantly trans in unfolded ribonuclease T1. This is shown by a comparison of the wild type and a designed mutant protein where Ser54 and Pro55 were replaced by Gly54 and Asn55, respectively. This mutation leaves the thermal stability of the protein almost unchanged; however, in the absence of Pro55 one of the two slow phases in folding is abolished and the kinetic mechanism of refolding is dramatically simplified.

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Citations

May 1, 1992·Protein Science : a Publication of the Protein Society·R RudolphT Kiefhaber
Jul 1, 1992·Protein Science : a Publication of the Protein Society·D A SchultzR L Baldwin
Sep 1, 1992·Protein Science : a Publication of the Protein Society·T KiefhaberA Chaffotte
Feb 1, 1994·Protein Science : a Publication of the Protein Society·L M MayrF X Schmid
Sep 1, 1994·Protein Science : a Publication of the Protein Society·M C ShastryJ B Udgaonkar
Jun 1, 1995·Protein Science : a Publication of the Protein Society·M H Zehfus
Jul 1, 1997·Protein Science : a Publication of the Protein Society·L S MullinsF M Raushel
Jul 1, 1997·Protein Science : a Publication of the Protein Society·J X Zhang, D P Goldenberg
Jun 1, 1992·Journal of Biotechnology·O LandtU Hahn
May 26, 1999·Molecular and Biochemical Parasitology·A P Page, A D Winter
Jun 15, 2011·Journal of the American Chemical Society·Caroline HauptJochen Balbach
Mar 1, 2013·Journal of the American Chemical Society·Gabriel ZoldákFranz X Schmid
Oct 27, 1998·Nature Biotechnology·V SieberF X Schmid
Sep 26, 1997·Nature Structural Biology·D K HeidaryP A Jennings
Nov 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Anna L MallamSophie E Jackson
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E R Schönbrunner, F X Schmid
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·A MatouschekG Schatz
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·S WalterF X Schmid
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Jan 18, 2005·Biological Chemistry·Hisae MatsuuraKenji Takahashi
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Lorenz RognoniMatthias Rief
Jun 4, 2013·Amino Acids·Pierrick CraveurJoseph Rebehmed
Oct 4, 2012·The Journal of Physical Chemistry. B·Esteban Vöhringer-MartinezAlejandro Toro-Labbé
Dec 6, 2014·Acta Crystallographica. Section D, Biological Crystallography·Alexander Ulrich, Markus C Wahl
Dec 15, 2010·Journal of Molecular Biology·Gabriel Zoldák, Franz X Schmid
Feb 1, 2008·Journal of Molecular Biology·Tobias Aumüller, Gunter Fischer
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Jan 11, 2014·Protein Science : a Publication of the Protein Society·David J RosenmanChristopher Bystroff
Nov 20, 1991·Journal of Molecular Biology·J Martinez-OyanedelW Saenger

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