Replacement of ether with alternate volatile anesthetics for collection of rat serum used in embryo culture

Toxicology in Vitro : an International Journal Published in Association with BIBRA
P D C Brown-WoodmanC Emmanuel

Abstract

The traditional anesthetic used for collection of the serum culture medium for whole rat embryo culture studies has been ether. However ethical concerns have been raised due to the irritant nature of the vapour and safety concerns due to the risk of fire. Growth and development of gestation day 9.5 rat embryos cultured for 48 h in serum collected from rats anesthetised with either ether, isoflurane or halothane were compared. There were no differences in any of the parameters used to assess embryonic development when embryos were grown in serum collected using either ether or isoflurane anesthetics. However, when embryos grown in serum collected using ether or halothane were compared, embryonic development was similar in all respects, except for a reduced number of embryos turned to become fully dorsally convex in the halothane group (p <0.05). The data indicate that isoflurane is an appropriate alternative to ether for collection of the serum culture medium for whole rat embryo culture, while halothane may cause some delay of embryonic development.

References

Dec 1, 1979·Acta Anaesthesiologica Scandinavica·S PopovaB Sahatchiev
Jul 1, 1976·Journal of Toxicology and Environmental Health·G L KennedyJ C Calandra
Oct 1, 1975·Anesthesiology·E N CohenE Watson
Sep 1, 1975·Anesthesiology·D A HoladayM A Zumbiel
Mar 11, 1976·Toxicology and Applied Pharmacology·G L KennedyJ C Calandra
Jan 1, 1990·International Archives of Occupational and Environmental Health·C Baeder, M Albrecht
Jan 1, 1974·International Anesthesiology Clinics·K Rehder, A D Sessler
Nov 1, 1968·Anesthesiology·A B Basford, B R Fink

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