Replacement of K+ with Rb+ or Cs+, and its effects on the mechanical responses to norepinephrine and methacholine in the rat vas deferens

European Journal of Pharmacology
N SuzukiH Fukuda

Abstract

The rat vas deferens was stored overnight in cold, K+-free Krebs solution to deplete intracellular K+ then incubated in K+-, Rb+-, or Cs+-containing Krebs solution at 37 degrees C to load these ions inside the cells. After 4 h, the contents of K+ or Rb+ reached the level of K+ in the fresh vas deferens; the content of Cs+ was less than half that of the fresh vas deferens. Dose-response curves to norepinephrine and methacholine were determined under these conditions, and the curves in Rb+ or Cs+ solution were compared with those in K+ solution. The cold storage per se had little effect on the dose-response curves in K+ solution except that it slightly decreased the maximal response to norepinephrine. The dose-response curves in Rb+ solution were to the left of those in K+ solution. The maximal response to methacholine was greatly increased. On the other hand, the dose-response curves in Cs+ solution were to the right of those in K+ solution. The maximal responses were greatly decreased with both drugs. The results suggest that Rb+ but not Cs+ can fully substitute for K+ in the rat vas deferens response to norepinephrine and methacholine.

References

Jan 1, 1975·Annual Review of Physiology·I M Glynn, S J Karlish
Apr 1, 1977·The Journal of Physiology·J H Widdicombe
Nov 1, 1975·Medical Hypotheses·P M Wiggins
Feb 1, 1971·Comparative Biochemistry and Physiology. A, Comparative Physiology·G S TaylorE E Daniel
Feb 1, 1971·The Journal of Physiology·T Tomita, T Yamamoto
May 1, 1961·The Journal of General Physiology·R A Sjodin

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