Replating of bioreactor expanded human bone marrow results in extended growth of primitive and mature cells.

Cytotechnology
B O PalssonM R Koller

Abstract

The capability to expand human bone marrow mononuclear cells (BM MNC) in high density perfusion culture chambers (bioreactors) has recently been developed. In these bioreactors, total cell colony-forming unit-granulocyte/macrophage (CFU-GM), and long-term culture-initiating cell (LTC-IC) numbers increase significantly over a 14-day period. However, cell growth ceases after the 14-day period, possibly due to cell density limitations. Because of the remaining presence of early cells, it should be feasible to replate the cells and obtain continued expansion. In this study, we demonstrate that bioreactors generate cells, which upon replating into secondary bioreactors, lead to continued cell, CFU-GM, and LTC-IC(8) (measured after 8 weeks of secondary culture) expansion. A two-stage protocol, involving the replating of cells on days 9 to 12 of culture into new bioreators at the original seeding density, yielded greater than 50-fold cell expansion from BM MNC in 25 days. CFU-GM were expanded inhibitory factor (LIF) had no significant effect on total cells, CFU-GM, or LTC-IC(5) in this system. We conclude that two-stage bioreactor cultures are capable of supporting extended growth of human BM MNC, CFU-GM, and LTC-IC(8). The continued ...Continue Reading

References

May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H J SutherlandC J Eaves
Mar 12, 1982·Journal of Immunological Methods· Fazekas de St Groth
Sep 1, 1993·The Journal of Experimental Medicine·P M LansdorpH Mayani
Oct 1, 1993·Biotechnology and Bioengineering·M R Koller, B O Palsson
Mar 1, 1951·The Journal of Hygiene·D J Finney

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