PMID: 2492507Feb 1, 1989Paper

Replication and segregational stability of Bacillus plasmid pBAA1

Journal of Bacteriology
K M DevineD J McConnell

Abstract

A cryptic plasmid, pBAA1, was identified in an industrial Bacillus strain. The plasmid is 6.8 kilobases in size and is present in cells at a copy number of approximately 5 per chromosome equivalent. The plasmid has been maintained under industrial fermentation conditions without apparent selective pressure and so is assumed to be partition proficient. The minimal replicon was localized to a 1.4-kilobase fragment which also contains the functions required for copy number control. The very low level of segregational instability of the minimal replicon suggests that it also contains functions involved in plasmid maintenance. Comparison with other plasmids indicates that pBAA1 belongs to the group of small gram-positive plasmids which replicate by a rolling cycle-type mechanism. A sequence was identified which is required for the efficient conversion of the single plus strand to the double-stranded form during plasmid replication. Deletion of this sequence resulted in a low level of segregational plasmid instability.

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Citations

Sep 18, 2015·The Journal of General and Applied Microbiology·Huanhuan ShaoHong Feng
Jan 1, 1993·Antonie van Leeuwenhoek·P H Pouwels, R J Leer
Mar 29, 2001·Journal of Bacteriology·L R BarranE S Bromfield
Aug 29, 2006·Applied and Environmental Microbiology·Mujin TangBrian A Federici
Jun 1, 1989·Microbiological Reviews·A Gruss, S D Ehrlich
Jun 1, 1989·Journal of Bacteriology·L BoeA Gruss
Jan 1, 1992·Journal of Bacteriology·E De RossiG Riccardi
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·G del SolarR Díaz-Orejas
Aug 1, 1995·Applied and Environmental Microbiology·S EbisuS Udaka
Jan 1, 1993·Applied and Environmental Microbiology·M Vujcic, L Topisirovic
Mar 1, 1990·Gene·L JannièreS D Ehrlich
Dec 31, 1997·Microbiology and Molecular Biology Reviews : MMBR·S A Khan

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