Replication at the telomeres of the Streptomyces linear plasmid pSLA2

Molecular Microbiology
Zhongjun Qin, Stanley N Cohen

Abstract

The Streptomyces linear plasmid pSLA2 initiates DNA replication bidirectionally towards its telomeres from a site located near the centre of the molecule; at the telomeres, the recessed ends of lagging strands are filled in by non-displacing DNA synthesis. Here, we report experiments that test three proposed mechanisms for lagging-strand fill-in. We present data inconsistent with recombinational or terminal hairpin models for the formation of full-length duplex pSLA2 DNA. Instead, we find that deletions in short, distantly separated homologous palindromes in the leading-strand 3' overhang prevent propagation of linear pSLA2 DNA, implicating a mechanism of palindrome-mediated leading-strand fold-back in telomere replication. We further show that circularized pSLA2 DNA molecules are opened in vivo precisely at the terminal nucleotides of telomeres, generating functional linear replicons containing native telomeres covalently bound to a protein at their 5' DNA termini. Together, our results support a model in which pairing of multiple widely separated pSLA2 palindromes anchors the 3' end of the leading-strand overhang to a site near the overhang's base -- providing a recognition site for terminal-protein-primed DNA synthesis and s...Continue Reading

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Citations

Sep 28, 2002·Trends in Genetics : TIG·Carton W ChenRalph Kirby
May 10, 2000·FEMS Microbiology Letters·J N Volff, J Altenbuchner
Jul 20, 2002·FEMS Microbiology Letters·Kerstin SpatzMatthias Redenbach
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Jul 26, 2002·Molecular Microbiology·Zhongjun Qin, Stanley N Cohen
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Sep 8, 2006·Applied and Environmental Microbiology·Ran ZhangZhongjun Qin
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May 20, 2004·Journal of Bacteriology·Tetsuya UchidaHaruyasu Kinashi
Jun 10, 2006·Annual Review of Genetics·David A Hopwood
Jul 2, 2003·Bioscience, Biotechnology, and Biochemistry·Shoichi InoueHaruyasu Kinashi
Dec 18, 2013·Applied Microbiology and Biotechnology·Maksym MyronovskyiAndriy Luzhetskyy
Sep 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Neal F Lue, Sulin Jiang
Jun 15, 2011·Bioscience, Biotechnology, and Biochemistry·Yingjie YangHaruyasu Kinashi
Sep 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kai Bao, Stanley N Cohen
Mar 8, 2003·Molecular Microbiology·Chih-Hung HuangCarton W Chen
Mar 21, 2007·Molecular Microbiology·Chih-Hung HuangCarton W Chen
Dec 1, 2004·Annual Review of Genetics·Stephen D Bentley, Julian Parkhill

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