Replication-competent recombinant porcine reproductive and respiratory syndrome (PRRS) viruses expressing indicator proteins and antiviral cytokines.

Viruses
Yongming SangFrank Blecha

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) can subvert early innate immunity, which leads to ineffective antimicrobial responses. Overcoming immune subversion is critical for developing vaccines and other measures to control this devastating swine virus. The overall goal of this work was to enhance innate and adaptive immunity following vaccination through the expression of interferon (IFN) genes by the PRRSV genome. We have constructed a series of recombinant PRRS viruses using an infectious PRRSV cDNA clone (pCMV-P129). Coding regions of exogenous genes, which included Renilla luciferase (Rluc), green and red fluorescent proteins (GFP and DsRed, respectively) and several interferons (IFNs), were constructed and expressed through a unique subgenomic mRNA placed between ORF1b and ORF2 of the PRRSV infectious clone. The constructs, which expressed Rluc, GFP, DsRed, efficiently produced progeny viruses and mimicked the parental virus in both MARC-145 cells and porcine macrophages. In contrast, replication of IFN-expressing viruses was attenuated, similar to the level of replication observed after the addition of exogenous IFN. Furthermore, the IFN expressing viruses inhibited the replication of a second PRRS viru...Continue Reading

References

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Citations

Jul 25, 2014·Journal of Virology·Yongming SangFrank Blecha
Nov 18, 2015·Virology Journal·Zhijun LiEn-Min Zhou
Mar 28, 2017·Veterinary Microbiology·Hanzhong Ke, Dongwan Yoo

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Datasets Mentioned

BETA
JN882576

Methods Mentioned

BETA
Infection
transfection
lavage
PCR

Software Mentioned

AlphaEase FC
Sigmaplot

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